0000000000352920

AUTHOR

J. C. Igual

showing 2 related works from this author

Respiration and low cAMP-dependent protein kinase activity are required for high-level expression of the peroxisomal thiolase gene in Saccharomyces c…

1996

Transcription of genes for peroxisomal proteins is repressed by glucose and induced by oleate. At least for the peroxisomal thiolase gene (POT1) there is a third regulatory mechanism, mediated by the transcription factor Adr1p, which is responsible for the high-level expression of the gene in stationary phase. Here we show that a region in the POT1 promoter that extends from positions -238 to -152 mediates this mechanism, and we suggest that Adr1p acts indirectly on POT1. We have also analyzed the role of the cAMP-dependent protein kinase (PKA) in the transcriptional regulation of POT1. PKA exerts a negative control: the high, unregulated PKA activity in a bcy1 mutant maintains POT1 transcr…

Regulation of gene expressionSaccharomyces cerevisiae ProteinsTranscription GeneticThiolaseSaccharomyces cerevisiaeBiologyRegulatory Sequences Nucleic AcidCAMP-dependent protein kinase activityCyclic AMP-Dependent Protein KinasesMicrobodiesMitochondriaDNA-Binding ProteinsFungal ProteinsBiochemistryRegulatory sequenceGene Expression Regulation FungalGeneticsTranscriptional regulationRas2Acetyl-CoA C-AcetyltransferaseProtein kinase APromoter Regions GeneticMolecular BiologyTranscription factorTranscription FactorsMoleculargeneral genetics : MGG
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ADR1 and SNF1 Mediate Different Mechanisms in Transcriptional Regulation of Yeast POT1 Gene

1994

We studied the consequences of adr1 and snf1 mutations on POT1 gene expression in different growth conditions. The results obtained reveal that ADR1 and SNF1 genes affect POT1 transcription in different ways: ADR1 has a minor role in derepression in low concentration of glucose but is essential for activation in stationary phase whereas SNF1 is essential for derepression and activation, although it does not seem to be directly involved in the molecular mechanism of activation in stationary phase.

Transcription GeneticRecombinant Fusion ProteinsGenes FungalBiophysicsSaccharomyces cerevisiaeBiologyMicrobodiesBiochemistryTranscription (biology)Gene Expression Regulation FungalGene expressionTranscriptional regulationAcetyl-CoA C-AcetyltransferaseLuciferasesMolecular BiologyGeneDerepressionRegulation of gene expressionGeneticsfungiGene Transfer TechniquesCell BiologyYeastCulture MediaCell biologycarbohydrates (lipids)GlucoseStationary phaseMutationProtein KinasesBiochemical and Biophysical Research Communications
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