0000000000353545
AUTHOR
Laura Alksne
Development of a Novel Method for Identification of Alaria alata Mesocercariae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
Alaria (A.) alata mesocercariae (AM) have increasingly appeared as incidental findings during the mandatory inspection of wild boars for Trichinella in many European countries. An Alaria spp.-specific PCR is available for the identification of AM
Growth and Antagonism of <i>Trichoderma</i> spp. and Conifer Pathogen <i>Heterobasidion annosum</i> s.l. <i>in Vitro</i> at Different Temperatures
Variations in the radial growth rate of 24 isolates belonging to ten species of Trichoderma, three isolates of conifer pathogen Heterobasidion annosum s.s. and four isolates of H. parviporum were evaluated by incubation on a solid malt extract medium at a temperature of 4℃, 15℃ and 21℃. Trichoderma antagonism against Heterobasidion was investigated in dual culture in vitro. The slowest rate of growth was referable to all seven strains of Heterobasidion spp. All Heterobasidion spp. strains were overgrown by 63% of Trichoderma spp. strains after two weeks at 21℃ and by 33% of strains at 15℃. 21% of Trichoderma strains did not grow and only four strains belonging to T. koningii, T. viride and …
Determination of Fungi and Multi-Class Mycotoxins in Camelia sinensis and Herbal Teas and Dietary Exposure Assessment
In this paper, a study of fungal and multi-mycotoxin contamination in 140 Camellia sinensis and 26 herbal teas marketed in Latvia is discussed. The analysis was performed using two-dimensional liquid chromatography with time-of-flight mass spectrometry (2D-LC-TOF-MS) and MALDI-TOF-MS. In total, 87% of the tea samples tested positive for 32 fungal species belonging to 17 genera, with the total enumeration of moulds ranging between 1.00 ×
Trichoderma spp. un Phlebiopsis gigantea ietekme uz Heterobasidion annosum s.l. augšanu egles un priedes koksnē laboratorijas apstākļos
Heterobasidion annosum s.l izraisītā sakņu trupe ir postoša skujkoku infekcija. Tās ierobežošanai izmanto Phlebiopsis gigantea suspensiju, taču arī Trichoderma spp. ir spēcīgi antagonisti. Mērķis bija novērtēt Trichoderma spp., P. gigantea un to kombinācijas iedarbību uz H. annosum s.l. Pētīta sēņu augšana uz priedes un egles bluķiem. Mikroskopēti krāsoti egles koksnes griezumi, noteikts koksnes ķīmiskais sastāvs, masas zudumi sēņu ietekmē un Trichoderma spp. aizsargefektivitāte. Konstatēts, ka visas antagonistisko sēņu suspensijas un to kombinācijas inhibēja H. annosum s.l. augšanu uz abu skujkoku virsmām. Trichoderma spp. aizsargefektivitāte pret H. annosum s.l. egles koksnes masas zudumi…
Prevalence, Genetic Diversity and Factors Associated with Distribution of Listeria monocytogenes and Other Listeria spp. in Cattle Farms in Latvia
Listeria spp. is a diverse genus of Gram-positive bacteria commonly present in the environment while L. monocytogenes and L. ivanovii are well known human and ruminant pathogens. The aim of the present study was to reveal the prevalence and genetic diversity of L. monocytogenes and other Listeria spp. and to identify the factors related to the abundance of pathogen at cattle farms. A total of 521 animal and environmental samples from 27 meat and dairy cattle farms were investigated and the genetic diversity of L. monocytogenes isolates was studied with WGS. The prevalence of Listeria was 58.9%, while of L. monocytogenes it was −11%. The highest prevalence of L. monocytogenes was found in th…