0000000000355354

AUTHOR

Gabriel Rutter

showing 3 related works from this author

Characterization of cell lines carrying self-replicating hepatitis C virus RNAs.

2001

ABSTRACT Subgenomic selectable RNAs of the hepatitis C virus (HCV) have recently been shown to self-replicate to high levels in the human hepatoma cell line Huh-7 (V. Lohmann, F. Körner, J. O. Koch, U. Herian, L. Theilmann, and R. Bartenschlager, Science 285:110–113, 1999). Taking advantage of this cell culture system that allows analyses of the interplay between HCV replication and the host cell, in this study we characterized two replicon-harboring cell lines that have been cultivated for more than 1 year. During this time, we observed no signs of cytopathogenicity such as reduction of growth rates or ultrastructural changes. High levels of HCV RNAs were preserved in cells passaged under…

Hepatitis C virusImmunoelectron microscopyImmunologyHepacivirusBiologyViral Nonstructural Proteinsmedicine.disease_causeVirus ReplicationMicrobiologyViral ProteinsVirologymedicineTumor Cells CulturedHumansRepliconPhosphorylationNS5ARNAVirologyMolecular biologyVirus-Cell InteractionsNS2-3 proteaseViral replicationCell cultureInsect ScienceRNA ViralRepliconJournal of virology
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Persistent and Transient Replication of Full-Length Hepatitis C Virus Genomes in Cell Culture

2002

ABSTRACT The recently developed subgenomic hepatitis C virus (HCV) replicons were limited by the fact that the sequence encoding the structural proteins was missing. Therefore, important information about a possible influence of these proteins on replication and pathogenesis and about the mechanism of virus formation could not be obtained. Taking advantage of three cell culture-adaptive mutations that enhance RNA replication synergistically, we generated selectable full-length HCV genomes that amplify to high levels in the human hepatoma cell line Huh-7 and can be stably propagated for more than 6 months. The structural proteins are efficiently expressed, with the viral glycoproteins E1 and…

ImmunologyReplicationGenome ViralHepacivirusBiologyVirus ReplicationMicrobiologyVirusViral ProteinsGene FrequencyVirologyTumor Cells CulturedHumansSubgenomic mRNAchemistry.chemical_classificationEndoplasmic reticulumRNAHepatitis CMolecular biologyNS2-3 proteasechemistryViral replicationCell cultureCulture Media ConditionedInsect ScienceRNA ViralGlycoproteinSubcellular FractionsJournal of Virology
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IFN? expression inhibits LHBs storage disease and ground glass hepatocyte appearance, but exacerbates inflammation and apoptosis in HBV surface prote…

2006

BACKGROUND/AIMS Interferon gamma (IFNgamma) controls hepatitis B virus replication. As systemic application may cause severe adverse effects, approaches of liver-directed IFNgamma gene therapy may represent an attractive alternative for treatment of chronic viral hepatitis B and thus needs testing in vivo in suitable animal models. METHODS We therefore crossbred Alb-1HBV transgenic mice overexpressing the large HBV surface protein (LHBs) in their livers and developing LHBs storage disease and ground glass hepatocyte appearance with SAP-IFNgamma transgenic animals previously shown to exhibit constitutive hepatic IFNgamma expression, and analyzed the resulting double-transgenic offspring. RES…

Genetically modified mouseHepatitis B virusHepatitis B Surface AntigensHepatologyGenetic enhancementTransgeneApoptosisMice TransgenicGround glass hepatocyteGenetic TherapyBiologymedicine.disease_causePeripheral blood mononuclear cellMice Inbred C57BLInterferon-gammaMiceHepatitis B ChronicLiverApoptosisImmunologyHepatocytesmedicineAnimalsInterferon gammamedicine.drugLiver International
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