0000000000356990

AUTHOR

Inmaculada Palacín

showing 4 related works from this author

Determining the relationship between bull sperm kinematic subpopulations and fluorescence groups using an integrated sperm quality analysis technique.

2017

The aim of the present study was to determine whether there is an association between the kinematic sperm subpopulations and fluorescent groups in bulls using a new fluorescent staining method that allows classification of spermatozoa into groups depending on their acrosomal and membrane integrity, as well as functional status, without inhibiting sperm motility. Cryopreserved semen samples from 10 Holstein bulls were used in the study. A multiparametric analysis of results obtained by the ISAS 3Fun kit (Proiser) was performed. The different fluorescent groups were detected and their motility characteristics evaluated using ISAS software. Clustering procedures using the kinematic data resul…

Maleendocrine systemMotilitySemenReproductive technologyBiologyCryopreservationFluorescenceAndrology03 medical and health sciences0302 clinical medicineEndocrinologyGeneticsAnimalsMolecular Biologyreproductive and urinary physiologySperm motility030219 obstetrics & reproductive medicineurogenital systemMultiparametric Analysis0402 animal and dairy science04 agricultural and veterinary sciences040201 dairy & animal scienceFluorescenceSpermSpermatozoaSemen AnalysisFertilityReproductive MedicineSperm MotilityAnimal Science and ZoologyCattleSoftwareDevelopmental BiologyBiotechnologyReproduction, fertility, and development
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Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls

2021

The objective of the present study was to investigate whether fertility differences in bulls are reflected in variations of sperm quality when analysing only one ejaculate per male. Two experiments were performed. In the first experiment, frozen semen samples from 20 adult bulls were tested

capacitationendocrine systemQH301-705.5Sperm Headanimal diseasesmedia_common.quotation_subjectAcrosome reaction<i>Bos taurus</i>acrosome reactionSemenFertilityBiologyArticlemale fertilityGeneral Biochemistry Genetics and Molecular BiologyAndrologyfluids and secretionsCapacitationBiology (General)Acrosomereproductive and urinary physiologySperm motilitymedia_commonGeneral Immunology and Microbiologyurogenital systemspermiogramSpermBos taurusGeneral Agricultural and Biological SciencesBiology
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A comparative study of the sperm nuclear morphometry in cattle, goat, sheep, and pigs using a new computer-assisted method (CASMA-F)

2012

This study was designed to compare the sperm nuclear morphometry of four species of domestic artiodactyls (cattle, sheep, goats, and pigs), using the newly developed automatic computer-assisted sperm morphometry analysis-F. The study was divided into two experiments. In the first experiment, samples from 20 males from each species were collected, diluted, and divided into four sample aliquots. The first was labeled directly with Hoechst 33342, and the others were processed as smears. Between smears, one group was directly labeled with Hoechst after air drying, and the other was fixed either with glutaraldehyde (GLUT), or with methanol, and afterward labeled with Hoechst. Digital images of t…

Maleendocrine systemBOARSwineSperm HeadSemenBiologyAndrologySpecies SpecificityFood AnimalsImage Processing Computer-AssistedAnimalsAir dryingSmall AnimalsFluorescent DyesCell NucleusSheepurogenital systemEquineGoatsAnatomySpermatozoaSpermOpen softwareMicroscopy FluorescenceBenzimidazolesCattleAnimal Science and ZoologyTheriogenology
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Predictive capacity of sperm quality parameters and sperm subpopulations on field fertility after artificial insemination in sheep

2015

This study was designed to evaluate the relevance of several sperm quality parameters and sperm population structure on the reproductive performance after cervical artificial insemination (AI) in sheep. One hundred and thirty-nine ejaculates from 56 adult rams were collected using an artificial vagina, processed for sperm quality assessment and used to perform 1319 AI. Analyses of sperm motility by computer-assisted sperm analysis (CASA), sperm nuclear morphometry by computer-assisted sperm morphometry analysis (CASMA), membrane integrity by acridine orange-propidium iodide combination and sperm DNA fragmentation using the sperm chromatin dispersion test (SCD) were performed. Clustering pro…

Maleendocrine systemmedicine.medical_treatmentmedia_common.quotation_subjectFertilitySemenSemen analysisBiologyLogistic regressionAndrologyEndocrinologyFood AnimalsPregnancymedicineAnimalsInsemination Artificialreproductive and urinary physiologySperm motilitymedia_commonSheepmedicine.diagnostic_testurogenital systemArtificial inseminationDomestic sheep reproductionGeneral MedicineSpermatozoaSpermSemen AnalysisFemaleAnimal Science and ZoologyAnimal Reproduction Science
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