0000000000359635

AUTHOR

Paola Rebuzzini

showing 3 related works from this author

Gatekeeper of pluripotency: A common Oct4 transcriptional network operates in mouse eggs and embryonic stem cells

2011

Abstract Background Oct4 is a key factor of an expanded transcriptional network (Oct4-TN) that governs pluripotency and self-renewal in embryonic stem cells (ESCs) and in the inner cell mass from which ESCs are derived. A pending question is whether the establishment of the Oct4-TN initiates during oogenesis or after fertilisation. To this regard, recent evidence has shown that Oct4 controls a poorly known Oct4-TN central to the acquisition of the mouse egg developmental competence. The aim of this study was to investigate the identity and extension of this maternal Oct4-TN, as much as whether its presence is circumscribed to the egg or maintained beyond fertilisation. Results By comparing …

Octamer Transcription Factor-3lcsh:QH426-470lcsh:BiotechnologycellsGene regulatory networkDown-RegulationBiologyTranscriptomeMicelcsh:TP248.13-248.65GeneticsInner cell massAnimalsGene Regulatory NetworksEmbryonic Stem Cellsreproductive and urinary physiologyOligonucleotide Array Sequence AnalysisGeneticsGene Expression ProfilingfungiEmbryoEmbryonic stem cellGene expression profilinglcsh:GeneticsMultigene FamilyCancer cellembryonic structuresOocytesFemalebiological phenomena cell phenomena and immunityFunction and Dysfunction of the Nervous SystemOctamer Transcription Factor-3Research ArticleBiotechnologyBMC Genomics
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Genome Stability in Embryonic Stem Cells

2011

Paola Rebuzzini1, Maurizio Zuccotti2*, Carlo Alberto Redi1,3 and Silvia Garagna1,4,5* 1Laboratorio di Biologia dello Sviluppo, Dipartimento di Biologia Animale, Universita degli Studi di Pavia, Via Ferrata 9, 27100 Pavia, 2Sezione di Istologia ed Embriologia, Dipartimento di Medicina Sperimentale, Universita degli Studi di Parma, Via Volturno 39, 43100 Parma 3Fondazione I.R.C.C.S. Policlinico San Matteo, Piazzale Golgi, 19, 27100 Pavia 4Centro di Ricerca Interdipartimentale di Ingegneria Tissutale, Universita degli Studi di Pavia, Via Ferrata 1, 27100 Pavia 5Centro di Eccellenza in Biologia Applicata, Universita degli Studi di Pavia, Via Ferrata 9, 27100 Pavia Italy

Homeobox protein NANOGCellular differentiationRex1Embryoid bodyStem cellBiologyInduced pluripotent stem cellMolecular biologyEmbryonic stem cellGenome stability
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Arsenic trioxide alters the differentiation of mouse embryonic stem cell into cardiomyocytes

2015

AbstractChronic arsenic exposure is associated with increased morbidity and mortality for cardiovascular diseases. Arsenic increases myocardial infarction mortality in young adulthood, suggesting that exposure during foetal life correlates with cardiac alterations emerging later. Here, we investigated the mechanisms of arsenic trioxide (ATO) cardiomyocytes disruption during their differentiation from mouse embryonic stem cells. Throughout 15 days of differentiation in the presence of ATO (0.1, 0.5, 1.0 μM) we analysed: the expression of i) marker genes of mesoderm (day 4), myofibrillogenic commitment (day 7) and post-natal-like cardiomyocytes (day 15); ii) sarcomeric proteins and their orga…

Fetal ProteinsSarcomeresMesodermTime FactorsCellular differentiationBlotting WesternConnexinFluorescent Antibody TechniqueGene ExpressionAntineoplastic AgentsActininBiologyArticleArsenicalsCell Linechemistry.chemical_compoundMiceArsenic TrioxideTroponin TSpheroids CellularGene expressionmedicineAnimalsActininMyocytes CardiacArsenic trioxideHomeodomain ProteinsSyncytiumMultidisciplinaryReverse Transcriptase Polymerase Chain ReactionCell DifferentiationMouse Embryonic Stem CellsOxidesEmbryonic stem cellCell biologyBiomechanical PhenomenaGATA4 Transcription Factormedicine.anatomical_structurechemistryConnexin 43ImmunologyHomeobox Protein Nkx-2.5T-Box Domain ProteinsTroponin CTranscription FactorsScientific Reports
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