0000000000365200

AUTHOR

Gian Marco Leggio

showing 4 related works from this author

Effects Of COOH-terminal tripeptide alpha-MSH (11-13) on corneal epithelial wound healing:role of nitric oxide

2006

It is known that alpha-melanocyte stimulating hormone (alpha-MSH) may exert anti-inflammatory effects and facilitate reparative processes in different tissues. The effective message sequence of alpha-MSH resides in the COOH-terminal tripeptide alpha-MSH(11-13). This study was undertaken to investigate the effects of topical administration of the COOH-terminal tripeptide sequence of alpha-MSH (alpha-MSH(11-13), KPV) on corneal epithelial wound healing in rabbits and the possible role of nitric oxide (NO) in these effects. The whole corneal epithelium was denuded in both eyes by mechanical abrasion. The area of the corneal epithelial defect was stained with fluorescein, photographed, and then…

MaleNitroprussideMelanocyte-Stimulating Hormonemedicine.medical_treatmentCorneal abrasionRabbitPharmacologyKPVNitric OxideNitric oxideCellular and Molecular Neurosciencechemistry.chemical_compoundPeptide Fragmentα-MSHmedicineAnimalsEnzyme InhibitorNitric Oxide DonorsMelanocyte-Stimulating HormonesFluoresceinEnzyme InhibitorsSalineCells CulturedCorneal epitheliumCell ProliferationEpithelial CellWound HealingbiologyDose-Response Relationship DrugAnimalcorneal wound healingEpithelium CornealEpithelial CellsNitric Oxide Donormedicine.diseaseSensory SystemsPeptide FragmentsNitric oxide synthaseOphthalmologymedicine.anatomical_structureNG-Nitroarginine Methyl EsterchemistryBiochemistrybiology.proteinSodium nitroprussideRabbitsWound healingmedicine.drug
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A novel arousal-based individual screening reveals susceptibility and resilience to PTSD-like phenotypes in mice

2021

Translational animal models for studying post-traumatic stress disorder (PTSD) are valuable for elucidating the poorly understood neurobiology of this neuropsychiatric disorder. These models should encompass crucial features, including persistence of PTSD-like phenotypes triggered after exposure to a single traumatic event, trauma susceptibility/resilience and predictive validity. Here we propose a novel arousal-based individual screening (AIS) model that recapitulates all these features. The AIS model was designed by coupling the traumatization (24 h restraint) of C57BL/6 J mice with a novel individual screening. This screening consists of z-normalization of post-trauma changes in startle …

Physiology5-trial SM 5-trial social memoryBiochemistryFight-or-flight responseFST forced swim test0302 clinical medicineEndocrinologySSRIs selective serotonin reuptake inhibitorsDSM-5 Diagnostic and Statistical Manual of Mental DisordersOriginal Research ArticleFear conditioningmedia_commonHT hypothalamusAIS arousal-based individual screeningQP351-495ParoxetinePhenotypeHPA hypothalamic–pituitary–adrenalBST basal synaptic transmissionHIP hippocampusPTSD post-traumatic stress disorder[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]Psychological resilienceAmy amygdalaRC321-571medicine.drugNeurophysiology and neuropsychologymedia_common.quotation_subjectBDNF brain derived neurotropic factorFear conditioningNeurosciences. Biological psychiatry. NeuropsychiatryBiologyStressArousal03 medical and health sciencesCellular and Molecular NeuroscienceAnimal model Fear conditioning Resilience Stress Susceptibility Z-scoreAnimal modelCORT corticosteroneOF open fieldTE trauma-exposedBiological neural networkmedicineAnimal model[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]C controlfEPSPs field excitatory post-synaptic potentialsSGK1 serum/glucocorticoid-regulated kinase 1RC346-429Molecular BiologyResilienceEndocrine and Autonomic SystemsZ-scoremPFC medial prefrontal cortexFKBP5 FK506 binding protein 5FDA Food and Drug AdministrationASR acoustic startle reactivityEPM elevated plus maze030227 psychiatrySusceptibilityAnimal model; Fear conditioning; Resilience; Stress; Susceptibility; Z-scoreNeurology. Diseases of the nervous systemNeuroscience030217 neurology & neurosurgeryNeurobiology of Stress
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Dopaminergic-GABAergic interplay and alcohol binge drinking

2019

© 2019 Elsevier Ltd The dopamine D 3 receptor (D 3 R), in the nucleus accumbens (NAc), plays an important role in alcohol reward mechanisms. The major neuronal type within the NAc is the GABAergic medium spiny neuron (MSN), whose activity is regulated by dopaminergic inputs. We previously reported that genetic deletion or pharmacological blockade of D 3 R increases GABA A α6 subunit in the ventral striatum. Here we tested the hypothesis that D 3 R-dependent changes in GABA A α6 subunit in the NAc affect voluntary alcohol intake, by influencing the inhibitory transmission of MSNs. We performed in vivo and ex vivo experiments in D 3 R knockout (D 3 R −/− ) mice and wild type littermates (D 3 …

0301 basic medicineMalemedicine.medical_specialtyDopaminergic-GABAergicSettore BIO/09 - FISIOLOGIAAlpha6 subunit; Dopamine D3 receptor; Ethanol; Furosemide (PubChem CID: 3440); GABA(A)receptor; Nucleus accumbens; Ro 15-4513; Ro 15-4513 (PubChem CID: 5081); SB 277011A (PubChem CID: 75358288)Alpha6 subunitNucleus accumbensMedium spiny neuronInhibitory postsynaptic potentialNucleus AccumbensBinge Drinking03 medical and health sciencesMiceDopamine D3 receptor0302 clinical medicineDopamine receptor D3Internal medicinemedicineAnimalsFurosemide (PubChem CID: 3440)Nucleus accumbenPharmacology & PharmacyRNA MessengerRo 15-4513GABAergic NeuronsSB 277011A (PubChem CID: 75358288).PharmacologyMice KnockoutEthanolGABAA receptorChemistryDopaminergicAntagonistReceptors Dopamine D3Receptors GABA-ARo 15-4513 (PubChem CID: 5081)GABA(A)receptor3. Good healthProtein Subunits030104 developmental biologyEndocrinologynervous systemGene Expression Regulation030220 oncology & carcinogenesisGABAergicNucleus accumbensSB 277011A (PubChem CID: 75358288)
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Hemin, an inducer of heme oxygenase-1, lowers intraocular pressure in rabbits.

2007

Carbon monoxide (CO) generated from heme may induce vasodilation and exert cyto-protective properties in the eye. This study was undertaken to investigate the effects of hemin, a potent inducer of heme oxygenase-1 (HO-1), on models of ocular hypertension in rabbits.Ocular hypertension was induced by injecting alpha-chymotrypsin in both eyes under local anesthesia. Only rabbits with an intraocular pressure (IOP) of 25 mmHg or more were used. The dose-response study of the hemin effect on IOP was made by an intravenous injection of the drug (50, 75, and 100 mg/kg) and subsequent IOP monitoring every 6 h. A separate set of animals was pretreated with the HO-1 inhibitor, zinc protoporphyrin-IX …

MaleIntraocular pressuregenetic structuresmedicine.drug_classOcular hypertensionProtoporphyrinsVasodilationPharmacologyBetamethasonechemistry.chemical_compoundRandom AllocationmedicineAnimalsChymotrypsinPharmacology (medical)Enzyme inducerIntraocular PressurePharmacologyAnalysis of VariancebiologyDose-Response Relationship Drugmedicine.diseaseeye diseasesHeme oxygenaseOphthalmologyDisease Models AnimalchemistryAnesthesiaEnzyme InductionInjections Intravenousbiology.proteinCorticosteroidBetamethasoneHeminOcular Hypertensionsense organsRabbitsHeme Oxygenase-1Heminmedicine.drugJournal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
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