0000000000367782

AUTHOR

Saverio Teresi

showing 5 related works from this author

Anti-actin antibodies in celiac disease: correlation with intestinal mucosa damage and comparison of ELISA with the immunofluorescence assay.

2005

The presence in the sera of celiac disease (CD) patients of anti-actin autoantibodies (AAAs) has been suggested as a marker of severe intestinal villus atrophy (1). AAAs have been detected with an immunofluorescence (IF) technique and seem to contribute to villus cytoskeleton damage and to the pathogenesis of intestinal damage in CD (2). The aims of the present study were to evaluate the relationship between the presence of serum IgA AAAs and severity of intestinal mucosa damage in CD patients and to compare the IF assay with a new ELISA for IgA AAA determination. We enrolled 150 individuals in the study. IgA AAAs were assayed in 58 consecutive CD patients diagnosed between January and Dece…

AdultMalemedicine.medical_specialtyPathologySettore MED/09 - Medicina InternaAdolescentClinical Biochemistryanti-actin autoantibodieFluorescent Antibody TechniqueEnzyme-Linked Immunosorbent AssayAutoimmune hepatitisGastroenterologyCoeliac diseasePrimary biliary cirrhosisIntestinal mucosaInternal medicinemedicineHumansIntestinal MucosaChildPediatric gastroenterologyAutoantibodiesbusiness.industryBiochemistry (medical)Intestinal villusAutoantibodyInfantMiddle Agedmedicine.diseaseActinsImmunoglobulin AFood intoleranceanti-actin autoantibodies; celiac disease; ELISAmedicine.anatomical_structureChild PreschoolFemaleELISAbusinessceliac disease
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Antiendomysium antibodies assay in the culture medium of intestinal mucosa: an accurate method for celiac disease diagnosis

2011

Background Celiac disease (CD) diagnosis is becoming more difficult as patients with no intestinal histology lesions may also be suffering from CD. Aim To evaluate the diagnostic accuracy of antiendomysium (EmA) assay in the culture medium of intestinal biopsies for CD diagnosis. Patients and methods The clinical charts of 418 patients with CD and 705 non-CD controls who had all undergone EmA assay in the culture medium were reviewed. Results EmA assay in the culture medium had a higher sensitivity (98 vs. 80%) and specificity (99 vs. 95%) than serum EmA/antibodies to tissue transglutaminase (anti-tTG) assay. All patients with CD who were tested as false-negatives for serum EmA and/or anti-…

AdultMalemedicine.medical_specialtyPathologySettore MED/09 - Medicina InternaAdolescentTissue transglutaminaseDuodenumBiopsyMuscle Fibers Skeletalceliac disease culture system diagnosis intestinal histology serum antiendomysiumDiseaseHuman leukocyte antigenGastroenterologyAntiendomysium antibodiesTissue Culture TechniquesYoung AdultIntestinal mucosaInternal medicineBiopsyMedicineHumansVillous atrophyIntestinal MucosaChildFalse Negative ReactionsAgedAutoantibodiesTransglutaminasesHepatologybiologymedicine.diagnostic_testbusiness.industryGastroenterologyInfantMiddle AgedCulture MediaCeliac DiseaseChild Preschoolbiology.proteinFemaleAntibodybusinessEpidemiologic MethodsBiomarkers
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Production of Anti-Endomysial Antibodies in Cultured Duodenal Mucosa: Usefulness in Coeliac Disease Diagnosis

2002

Although anti-endomysial antibodies (EmA) have been found in the supernatants of cultured intestinal mucosa from patients with coeliac disease (CD), in no study has the clinical reliability of this new diagnostic tool been investigated. Our aims were to evaluate the clinical usefulness of the in vitro production of EmA in CD diagnosis in consecutive patients with suspected CD, and to evaluate the reliability of the in vitro challenge in CD patients on a gluten-free diet (GFD).For the former aim, consecutive patients who were due to undergo intestinal biopsy for suspected diagnosis of CD were enrolled: according to the final diagnosis, these patients were divided into two groups: Group 1 com…

AdultMaleanti-endomysial antibodiemedicine.medical_specialtySettore MED/09 - Medicina InternaAdolescentDuodenumIn Vitro TechniquesSensitivity and SpecificityGastroenterologyGliadinCoeliac diseaseIntestinal mucosaImmunopathologyInternal medicineSuspected diagnosisHumansMedicineChildCells CulturedAgedbiologybusiness.industryGastroenterologyInfantReproducibility of ResultsMiddle Agedcultured duodenal mucosa: coeliac disease diagnosismedicine.diseaseAntibodies Anti-IdiotypicCeliac Diseasemedicine.anatomical_structureGastric MucosaChild PreschoolAntibody FormationDuodenumbiology.proteinDuodenal mucosaFemaleHistopathologyAntibodycoeliac disease diagnosis [anti-endomysial antibodies; cultured duodenal mucosa]businessScandinavian Journal of Gastroenterology
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Diagnostic efficacy of the ELISA test for the detection of deamidated anti-gliadin peptide antibodies in the diagnosis and monitoring of celiac disea…

2009

Background and Aim: We evaluated the diagnostic performance of an ELISA test for anti-gliadin IgA and IgG antibodies, which uses synthetic deamidated gliadin peptides (anti-gliadin antibodies, AGAs) as coating; the results were compared with a test that uses extracted gliadin (AGAe). Methods: The study was conducted on the sera of 144 patients suffering from celiac disease (CD), including 20 patients with IgA deficiency and 9 who were following a gluten-free diet (GFD), and 129 controls. Results: In the 115 CD patients (without IgA deficiency), the sensitivity of AGAe IgA and IgG was 32.2 and 60.9%, whereas that of AGAs IgA and IgG was 59.1 and 72.2%. The specificity for AGAe IgA and IgG, a…

MaleSettore MED/09 - Medicina InternaTissue transglutaminaseClinical BiochemistryGliadinSerologyImmunology and AllergyMedicinedeamidated anti-gliadin peptide antibodieChildFalse Negative Reactionsreproductive and urinary physiologybiologyHematologyMiddle Agedfemale genital diseases and pregnancy complicationsMedical Laboratory TechnologyChild PreschoolAnti-transglutaminase antibodiesAnti-gliadin antibodiesELISAFemaleAntibodyMicrobiology (medical)AdultAdolescenteducationEnzyme-Linked Immunosorbent AssaySensitivity and SpecificityAntibodiesYoung AdultAntigenELISA; deamidated anti-gliadin peptide antibodies; celiac diseaseHumansFalse Positive ReactionsSerologic TestsAgedAutoantibodiesTransglutaminasesbusiness.industryBiochemistry (medical)Public Health Environmental and Occupational HealthAutoantibodyOriginal ArticlesImmunoglobulin Abody regionsCeliac DiseaseROC CurveCase-Control StudiesImmunoglobulin GImmunologybiology.proteinbusinessGliadinPeptides
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IgA anti-actin antibodies ELISA in coeliac disease: A multicentre study.

2007

Previous studies have demonstrated that serum anti-actin antibodies are a reliable marker of intestinal damage severity in coeliac disease.To validate in a multicentre study the clinical usefulness of serum IgA anti-actin antibody ELISA and its possible use in monitoring intestinal mucosa lesions during gluten-free diet.Four centres recruited 205 newly diagnosed coeliac disease patients with villous atrophy, 80 healthy controls and 81 "disease" controls. Twelve coeliac disease patients on gluten-free diet but with persistent symptoms underwent serum IgA anti-actin antibody assay and intestinal histology evaluation. IgA anti-actin antibody ELISA was performed with a commercial kit. All coeli…

AdultMalePathologymedicine.medical_specialtySettore MED/09 - Medicina InternaAdolescentEnzyme-Linked Immunosorbent AssaySerum igaDiseaseCommercial kitSensitivity and SpecificityCoeliac diseaseIgA anti-actin antibodies; coeliac disease; multicentre studyIntestinal mucosaHumansMedicineIntestinal MucosaVillous atrophyChildAgedAutoantibodiesHepatologybiologybusiness.industryGastroenterologyInfantnutritional and metabolic diseasesMiddle AgedIgA anti-actin antibodiemedicine.diseaseActinsmulticentre studyImmunoglobulin ACeliac DiseaseIntestinal histologyChild Preschoolbiology.proteinFemaleAntibodybusinessBiomarkerscoeliac disease
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