0000000000368423
AUTHOR
Hansjürgen Bratzke
Isolation and molecular characterization of brain microvascular endothelial cells from human brain tumors.
Brain tumor formation and growth is accompanied by the proliferation and infiltration of blood capillaries. The phenotypes of endothelial cells that make up capillaries are known to differ not only in the tissues in which endothelial cells are located but also as a result of the microenvironment to which they are exposed. For this reason, primary cultures of brain endothelial cells were isolated from human brain tumors removed by surgery and compared with cells from normal tissue. The primary confluent monolayers that grew out of isolated capillary fragments consisted of closely associated, elongated, fusiform-shaped cells. But brain tumor-derived endothelial cells in culture exhibited sign…
Zur forensischen Beweisführung bei einer Silikonembolie durch Kombination histologischer und toxikologischer Untersuchungsmethoden
Am Beispiel eines Falles aus dem Obduktionsgut des rechtsmedizinischen Institutes Frankfurt werden diagnostische Moglichkeiten zum Nachweis einer Silikonembolie aufgezeigt. Es handelt sich um den Fall einer 21 Jahre alten Frau, die nach kosmetischen Injektionen von flussigem Silikon verstarb. Die histologischen Befunde (lichtoptisch leere Hohlraume im Weichteilgewebe des Gesases mit umgebender Zellreaktion, leere dilatierte Blutgefase in den Lungen sowie leere Blutgefase in den Glomerula der Nieren) und die Resultate der toxikologischen Untersuchungen (NMR-spektroskopischer und massenspektrometrischer Nachweis von Silikon im Nieren- und Lungengewebe) erlaubten es, in ihrer Kombination eine …
Tödliche Hundebisse im Sektionsgut rechtsmedizinischer Institute
Hundebisse werden im angelsachsischen Raum als ernstes medizinisches, soziales und legislatorisches Problem angesehen, wohingegen in der deutschsprachigen Literatur nahezu keine Angaben uber die Inzidenz derartiger, insbesondere letal ausgehender Verletzungen zu finden sind. Zur Gewinnung von Daten uber todliche Hundebiss-Vorfalle in einem ungefahr 10 Jahre umfassenden Zeitraum wurde ein Fragebogen an alle rechtsmedizinischen Institute in Deutschland, der Schweiz und Osterreich versandt. Aus 13 Instituten wurden 20 Falle berichtet, unter denen 11-mal als Todesursache Verbluten und 3-mal eine schwere Infektion genannt wurden. Funfmal war es zu einem Schadel-Hirn-Trauma und 7-mal zu einer Hal…
Strontium90 for determination of time since death
Strontium90 (Sr90) is an artificial nuclear fission product of the atmospheric a-bomb testing between 1945 and 1979. It was spread throughout the atmosphere in the following years. Sr90 is an analogue to calcium and therefore enriched in human bones. Several studies especially in the 1960s and 1970s were undertaken to investigate the Sr90 burden and the resulting incorporated radiation in humans, but present studies are missing. In this study nine bone samples, three from 1931/32 and six from 1989 to 1994 were examined by measuring the Sr90 radiation. The samples from 1931/32 did not show any Sr90 activity. All the samples from 1989 and later showed a Sr90 activity, but the intensity was ve…
Morphometric assessment of testicular changes in drug-related fatalities.
Testicular sections of 50 deceased drug addicts were examined by light microscopy using morphometric methods and determining the extent of spermatogenesis by application of the Johnsen score count. Twenty cases of violent death served as reference sample. The study was aimed at obtaining data about the degree of germinal gland damage following the duration of the individual drug career, which was correlated to the histologic findings. Progressively reduced maturation of germinal cells, decrease of tubular diameter and height of sperm-producing epithelium were found the longer the individual case history has been. The results clearly indicated a substantial impairment of male fertility in th…
Death caused by a chain saw – homicide, suicide or accident?
A 31-year-old farm worker was found dead beside a chain saw. Based on the testimony of a colleague and because of the situation at the site, the police very soon presupposed an accident. It was assumed that the victim had slipped on the sodden ground and had been killed by the subsequent swerving of the saw when starting the engine. The body presented with a deep lacerated wound at the right side of the nape of the neck, including the first cervical vertebra, the medullary canal and the right mandible as well as multiple fissures of the occipital bone, which were attributed to repeated forceful use of the chain by another person. A second wound with relatively sharp edges and a tangential f…