0000000000370969

AUTHOR

S Pereira

showing 6 related works from this author

Application of patient derived liver cancer cell lines for phenotypic characterization and therapeutic target identification

2018

Cell cultureGastroenterologymedicineIdentification (biology)Computational biologyBiologyLiver cancermedicine.diseasePhenotypeZeitschrift für Gastroenterologie
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Severe metabolic alterations in liver cancer lead to ERK pathway activation and drug resistance

2020

Background: The extracellular signal-regulated kinase (ERK) pathway regulates cell growth, and is hyper-activated and associated with drug resistance in hepatocellular carcinoma (HCC). Metabolic pathways are profoundly dysregulated in HCC. Whether an altered metabolic state is linked to activated ERK pathway and drug response in HCC is unaddressed. Methods: We deprived HCC cells of glutamine to induce metabolic alterations and performed various assays, including metabolomics (with 13C-glucose isotope tracing), microarray analysis, and cell proliferation assays. Glutamine-deprived cells were also treated with kinase inhibitors (e.g. Sorafenib, Erlotinib, U0126 amongst other MEK inhibitors). …

Life sciences; biology0301 basic medicineSorafenibMAPK/ERK pathwayCarcinoma HepatocellularResearch paperMAP Kinase Signaling SystemGlutamineProliferationlcsh:MedicineAntineoplastic AgentsGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciences0302 clinical medicineDownregulation and upregulationddc:570medicineSerineHumansHCCProtein Kinase InhibitorsCell Proliferationlcsh:R5-920Cell growthChemistryKinaseMicroarray analysis techniquesLiver Neoplasmslcsh:RGeneral MedicineHep G2 Cellsdigestive system diseasesMetabolic pathway030104 developmental biologyAnaerobic glycolysisDrug Resistance NeoplasmKinase inhibitors030220 oncology & carcinogenesisCancer researchMetabolomeMetabolic stateAerobic glycolysisTranscriptomelcsh:Medicine (General)medicine.drugEBioMedicine
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Application of Patient-Derived Liver Cancer Cells in Personalized Treatment Approach: Phenotypic Characterization and Therapeutic Target Identificati…

2018

business.industryPersonalized treatmentGastroenterologyMedicineIdentification (biology)Computational biologybusinessLiver cancermedicine.diseasePhenotypeZeitschrift für Gastroenterologie
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Differential effect of TGF-β family members on proliferation and migration in primary liver cancer

2019

business.industryCancer researchMedicinePrimary liver cancerbusinessDifferential (mathematics)Transforming growth factor35. Jahrestagung der Deutschen Arbeitsgemeinschaft zum Studium der Leber
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Targeting tumor-initiating cells and compensatory YAP pathway to overcome sorafenib resistance in hepatocellular carcinoma

2021

business.industryHepatocellular carcinomaCancer researchMedicinebusinessmedicine.diseaseTumor Initiating CellsSorafenib resistanceViszeralmedizin 2021 Gemeinsame Jahrestagung Deutsche Gesellschaft für Gastroenterologie, Verdauungs- und Stoffwechselkrankheiten (DGVS), Sektion Endoskopie der DGVS, Deutsche Gesellschaft für Allgemein und Viszeralchirurgie (DGAV)
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Application of patient-derived liver cancer cells for phenotypic characterization and therapeutic target identification.

2018

Primary liver cancer (PLC) ranks among the most lethal solid cancers worldwide due to lack of effective biomarkers for early detection and limited treatment options in advanced stages. Development of primary culture models that closely recapitulate phenotypic and molecular diversities of PLC is urgently needed to improve the patient outcome. Long-term cultures of 7 primary liver cancer cell lines of hepatocellular and cholangiocellular origin were established using defined culture conditions. Morphological and histological characteristics of obtained cell lines and xenograft tumors were analyzed and compared to original tumors. Time course analyses of transcriptomic and genomic changes were…

Cancer ResearchCarcinogenesisDNA Mutational AnalysisPrimary Cell CultureAntineoplastic AgentsDiseaseBiologymedicine.disease_causeTranscriptome03 medical and health sciencesMice0302 clinical medicineCell Line TumormedicineBiomarkers TumorAnimalsHumansPrecision MedicineDrug discoveryGene Expression ProfilingLiver NeoplasmsHigh-Throughput Nucleotide SequencingGenomicsPrecision medicinemedicine.diseasePhenotypeXenograft Model Antitumor AssaysOncologyCell culture030220 oncology & carcinogenesisMutationCancer researchKRASLiver cancerInternational journal of cancer
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