0000000000372563
AUTHOR
Patricia Okwieka
Carbohydrate-Based Nanocarriers Exhibiting Specific Cell Targeting with Minimum Influence from the Protein Corona.
Whenever nanoparticles encounter biological fluids like blood, proteins adsorb on their surface and form a so-called protein corona. Although its importance is widely accepted, information on the influence of surface functionalization of nanocarriers on the protein corona is still sparse, especially concerning how the functionalization of PEGylated nanocarriers with targeting agents will affect protein corona formation and how the protein corona may in turn influence the targeting effect. Herein, hydroxyethyl starch nanocarriers (HES-NCs) were prepared, PEGylated, and modified on the outer PEG layer with mannose to target dendritic cells (DCs). Their interaction with human plasma was then s…
Aggregation Behavior of Polystyrene-Nanoparticles in Human Blood Serum and its Impact on the in vivo Distribution in Mice
The interactions between nanoparticles (NPs) and proteins in complex biological application media such as blood serum are capable of inducing aggregate formation which can lead to subsequent changes in biological activity. Here, we correlate surface charge, aggregation-tendency, and surface serum protein adsorption with cellular uptake and biodistribution in mice. Polystyrene-based NPs (80 - 170 nm) with different surface functionalizations were synthesized and incubated with human serum. Interaction of NPs with serum proteins and aggregate formation were analyzed by mass spectrometryanalysis and dynamic light-scattering. Influence of surface functionalization on specific cellular uptake an…
Tailoring the stealth properties of biocompatible polysaccharide nanocontainers.
Fundamental development of a biocompatible and degradable nanocarrier platform based on hydroxyethyl starch (HES) is reported. HES is a derivative of starch and possesses both high biocompatibility and improved stability against enzymatic degradation; it is used to prepare nanocapsules via the polyaddition reaction at the interface of water nanodroplets dispersed in an organic miniemulsion. The synthesized hollow nanocapsules can be loaded with hydrophilic guests in its aqueous core, tuned in size, chemically functionalized in various pathways, and show high shelf life stability. The surface of the HES nanocapsules is further functionalized with poly(ethylene glycol) via different chemistri…
Kohlenhydrat-basierte Nanocarrier mit spezifischem Zell-Targeting und minimalem Einfluss durch die Proteinkorona
Sobald Nanopartikel mit biologischen Flussigkeiten wie Blut in Kontakt kommen, adsorbieren Proteine auf ihrer Oberflache, welche die sogenannte Proteinkorona ausbilden. Die Wichtigkeit dieser Proteinhulle ist weitgehend anerkannt, jedoch untersuchen nur wenige Studien den Einfluss von Oberflachenfunktionalisierung der Nanocarrier auf die Proteinkorona. Vor allem die Variation der Proteinkorona von PEGylierten und zusatzlich mit Targeting-Molekulen versehenen Nanotragern und der Einfluss auf das Targeting sind nicht bekannt. Hydroxyethylstarke-Nanocarrier (HES-NCs) wurden synthetisiert, anschliesend PEGyliert und zusatzlich (“on top”) mit Mannose funktionalisiert, um dendritische Zellen (DCs…