0000000000375413
AUTHOR
M. M. Barreca
Extracellular vesicles from CS fluid are possible biomarkers of multiple sclerosis disease status
Multiple sclerosis (MS) is an autoimmune disease and several researches have been focused on identifying molecular biomarkers that could reflect its heterogeneous clinical course and determine the best treatment option for patients. Some studies identified extracellular vesicles (EVs) in both the cerebrospinal fluid (CSF) and also in blood samples of patients affected by inflammatory neurodegenerative diseases and multiple sclerosis. To monitor the disease onset and its progression we compared the amount of CSF-EVs and their surface markers in subjects affected by either MS or other neurological disorders correlating the EVs with clinical indicators of MS disease severity. EVs isolated by u…
H2O2 INDUCES NECROPTOSIS IN MESOANGIOBLAST STEM CELLS
Stem cells are used in regenerative medicine, but their therapeutic efficacy is compromised by their huge death during the first days post-transplantation. Indeed, the microenvironment within damaged tissues is hostile for stem cell survival mainly due to oxidative stress. H2O2 may play a relevant role in inducing death of the injected cells. The aim of our study was to determine the mechanism of mesoangioblast (A6) cell death after an H2O2 treatment. FACS analysis with annV/PI showed that H2O2 induced a dose and time-dependent decrement in A6 viability. We have also found an increase in caspases 8, 9 and 3 activity after the treatment. To assess their involvement in cell death, the pan cas…
Extracellular vesicles shedded by mouse mesoangioblasts have paracrine effects on different cell types
Introduction Mouse mesoangioblasts are vessel associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. We have already demonstrated that these stem cells, as all the other stem cells, are able to release in the extracellular milieu membrane vesicles (EV) containing biological active molecules, such as FGF2, MMP2/91 and Hsp702. Today takes hold the idea that the vesicles can replace stem cells opening a new scenario in regenerative medicine3. To this aim, we investigated the possible paracrine interaction of mesoangioblast EV on different cell types and their effects. Results and conclusions We have showed that mesoangioblast EV interact with human e…