0000000000378969

AUTHOR

Henric S. Adler

showing 4 related works from this author

MAP kinase p38 and its relation to T cell anergy and suppressor function of regulatory T cells

2008

Diverse regulatory T cell populations (Treg) are important for the control of self tolerance and immune homeostasis. These include naturally occurring CD4+CD25+ Treg (nTreg) and induced Treg (iTreg). Tolerogenic dendritic cells, modulated by IL-10, are able to convert peripheral T cells into iTreg. These are anergic and characterized by a G(1) cell cycle arrest, dependent on elevated levels of the cdk inhibitor p27(Kip1). Novel data revealed a distinct pattern of MAP kinase activation in iTreg different from clonal T cell anergy, with enhanced activation of the p38-MAPKAP-K2/3 pathway. p38 is involved in cell cycle control and its activity is a prerequisite for the induction and maintenance…

Clonal AnergyCell cycle checkpointClonal anergyRegulatory T cellT cellCell CycleCell BiologyBiologyCell cycleT-Lymphocytes Regulatoryp38 Mitogen-Activated Protein KinasesCell biologymedicine.anatomical_structuremedicineAnimalsHumansCytotoxic T cellIL-2 receptorAntigen-presenting cellMolecular BiologyCyclin-Dependent Kinase Inhibitor p27Developmental BiologyCell Cycle
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Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut

2013

Background IgE- and T-cell cross-reactivity contribute to the birch pollen–food syndrome. Objectives We performed a comprehensive analysis of T-cell cross-reactivity in primary cell cultures, facilitating the identification of allergen-specific T-cell subpopulations from individual patients. Methods Patients with birch pollen allergy and associated food allergy to hazelnuts, carrots, or both were analyzed for IgE cross-reactivity, T-cell responses, and T-cell cross-reactivity to recombinant Bet v 1.0101 (Bet v 1; birch), Cor a 1.0401 (Cor a 1; hazelnut), and Dau c 1.0104 (Dau c 1; carrot). A novel flow cytometry–based method using a 2-step staining process with fluorescent dyes was establis…

AdultMaleAllergyImmunologyCross ReactionsImmunoglobulin EFlow cytometryYoung Adultchemistry.chemical_compoundCorylusOral allergy syndromeAntigenT-Lymphocyte SubsetsFood allergymedicineHumansImmunology and AllergyBetulaCells CulturedPlant Proteinsmedicine.diagnostic_testbiologyChemistryfood and beveragesCarboxyfluorescein succinimidyl esterDendritic cellAllergensAntigens PlantImmunoglobulin EMiddle Agedmedicine.diseaseDaucus carotaCase-Control StudiesImmunologybiology.proteinPollenFemaleFood HypersensitivityJournal of Allergy and Clinical Immunology
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Neuronal nitric oxide synthase modulates maturation of human dendritic cells.

2010

AbstractDendritic cells (DCs) are the most potent APCs of the immune system. Understanding the intercellular and intracellular signaling processes that lead to DC maturation is critical for determining how these cells initiate T cell-mediated immune processes. NO synthesized by the inducible NO synthase (iNOS) is important for the function of murine DCs. In our study, we investigated the regulation of the arginine/NO-system in human monocyte-derived DCs. Maturation of DCs induced by inflammatory cytokines (IL-1β, TNF, IL-6, and PGE2) resulted in a pronounced expression of neuronal NOS (nNOS) but only minimal levels of iNOS and endothelial NOS were detected in human mature DCs. In addition, …

T cellCellular differentiationImmunologyImmunoblottingchemical and pharmacologic phenomenaEnzyme-Linked Immunosorbent AssayCell SeparationNitric Oxide Synthase Type IBiologyEndothelial NOSLymphocyte ActivationNitric OxideProinflammatory cytokineCell LineImmune systemmedicineImmunology and AllergyHumansAutocrine signallingMHC class IIReverse Transcriptase Polymerase Chain ReactionCell DifferentiationDendritic CellsFlow CytometryCell biologymedicine.anatomical_structureCell culturebiology.proteinCytokinesJournal of immunology (Baltimore, Md. : 1950)
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Activation of MAP kinase p38 is critical for the cell-cycle–controlled suppressor function of regulatory T cells

2007

AbstractRegulatory T cells play an essential role in the control of self-tolerance and processes of adaptive immunity. Tolerogenic IL-10–modulated human dendritic cells (IL-10DCs) induce anergic T cells with strong suppressive properties (iTregs) that inhibit the activation of effector T cells. In this study, we evaluated the interaction between cell-cycle regulation and intracellular signaling in these iTregs. Analysis of signal transduction events revealed a down-regulation of the mitogen-activated protein kinases (MAPKs) Jun N-terminal kinase (JNK) and a nonactivation of extracellular-signal–regulated kinase (ERK) in contrast to a marked activation of p38 MAPK and the p38 effector MAPK-a…

MAPK/ERK pathwayp38 mitogen-activated protein kinasesImmunologyIn Vitro TechniquesProtein Serine-Threonine KinasesBiologyT-Lymphocytes Regulatoryp38 Mitogen-Activated Protein KinasesBiochemistryAldesleukinHumansProtein kinase AMitogen-Activated Protein Kinase KinasesKinaseCell CycleIntracellular Signaling Peptides and ProteinsJNK Mitogen-Activated Protein KinasesCell BiologyHematologyAcquired immune systemInterleukin-10Cell biologyMitogen-activated protein kinasebiology.proteinSignal transductionCyclin-Dependent Kinase Inhibitor p27Blood
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