0000000000379843

AUTHOR

Charlotte Dabat-blondeau

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3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

2013

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

Chemical imagingmedicine.medical_specialtyMaterials scienceInfrared spectroscopyBiochemistryFourier transform spectroscopyPhysics::GeophysicsMicesymbols.namesakeImaging Three-DimensionalOpticsSpectroscopy Fourier Transform InfraredMicroscopyImage Processing Computer-AssistedmedicineAnimalsHumansFourier transform infrared spectroscopyMolecular BiologyEmbryonic Stem Cellsbusiness.industryX-Ray MicrotomographyCell BiologyWoodSpectral imagingPopulusFourier transformsymbolsbusinessChemical fingerprintingSynchrotronsHairBiotechnologyNature Methods
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