3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

6533b7dbfe1ef96bd1270ba7

RESEARCH PRODUCT

3D spectral imaging with synchrotron Fourier transform infrared spectro-microtomography

Carol J. Hirschmugl Jonathan M. Castro Michael J. Nasse Charlotte Dabat-blondeau Michael C. Martin Hans A. Bechtel David Buschke Barbara L. Illman Barbara L. Illman Brenda M. Ogle Miriam Unger Julia Sedlmair Julia Sedlmair Marco Keiluweit Dilworth Y. Parkinson

subject

Chemical imaging medicine.medical_specialty Materials science Infrared spectroscopy Biochemistry Fourier transform spectroscopy Physics::Geophysics Mice symbols.namesake Imaging Three-Dimensional Optics Spectroscopy Fourier Transform Infrared Microscopy Image Processing Computer-Assisted medicine Animals Humans Fourier transform infrared spectroscopy Molecular Biology Embryonic Stem Cells business.industry X-Ray Microtomography Cell Biology Wood Spectral imaging Populus Fourier transform symbols business Chemical fingerprinting Synchrotrons Hair Biotechnology

description

We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).

year	journal	country	edition	language
2013-08-04	Nature Methods

https://doi.org/10.1038/nmeth.2596

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