Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
All authors: Andrea Cossarizza Hyun‐Dong Chang Andreas Radbruch Andreas Acs Dieter Adam Sabine Adam‐Klages William W. Agace Nima Aghaeepour Mübeccel Akdis Matthieu Allez Larissa Nogueira Almeida Giorgia Alvisi Graham Anderson Immanuel Andrä Francesco Annunziato Achille Anselmo Petra Bacher Cosima T. Baldari Sudipto Bari Vincenzo Barnaba Joana Barros‐Martins Luca Battistini Wolfgang Bauer Sabine Baumgart Nicole Baumgarth Dirk Baumjohann Bianka Baying Mary Bebawy Burkhard Becher Wolfgang Beisker Vladimir Benes Rudi Beyaert Alfonso Blanco Dominic A. Boardman Christian Bogdan Jessica G. Borger Giovanna Borsellino Philip E. Boulais Jolene A. Bradford Dirk Brenner Ryan R. Brinkman Anna E. S. Broo…
Guidelines for the use of flow cytometry and cell sorting in immunological studies
The marriage between immunology and cytometry is one of the most stable and productive in the recent history of science. A rapid search in PubMed shows that, as of July 2017, using “flow cytometry immunology” as a search term yields more than 68 000 articles, the first of which, interestingly, is not about lymphocytes. It might be stated that, after a short engagement, the exchange of the wedding rings between immunology and cytometry officially occurred when the idea to link fluorochromes to monoclonal antibodies came about. After this, recognizing different types of cells became relatively easy and feasible not only by using a simple fluorescence microscope, but also by a complex and some…
Control of Exchange Interactions in Trinuclear Complexes Based on Orthogonal Magnetic Orbitals
The reaction of copper(II) acetate with the tetradentate Schiff base like ligand H4L {(E,E)-[{diethyl 2,2′-[4,5-dihydroxy-1,2-phenylenebis(iminomethylidyne)]bis(3-oxobutanoate)}] leads to the formation of the square planar N2O2 coordinated complex [H2CuL]. Reaction of two equivalents of this complex with copper(II) acetylacetonato or vanadyl(IV) acetylacetonato yields the trinuclear complexes [V(O)Cu2L2][N(nBu)4]2·2MeOH (1) and [Cu3L2][N(nBu)4]2·2DMF (2). Both complexes were characterised by using magnetic measurements and X-ray crystallography. Special attention was given to the spin-exchange coupling through the bridging phenylene ring. The principle of strict orthogonality of the magneti…