6533b86efe1ef96bd12cbfab
RESEARCH PRODUCT
Guidelines for the use of flow cytometry and cell sorting in immunological studies
Guadalupe HerreraJens GeginatDaryl GrummittVincenzo BarnabaJoanne LanniganBeate RückertElisabetta TraggiaiChristian MünzSusanne MelzerAri WaismanPratip K. ChattopadhyayJonas HahnT. Vincent ShankeyS SchmidJulia TornackDavid W. HedleyPaolo DellabonaJürgen WienandsAna CumanoEster B. M. RemmerswaalChristopher A. HunterVan Duc DangAnis LarbiTimothy P. BushnellMor GrossWenjun OuyangVera S. DonnenbergLilly LopezHolden T. MaeckerJenny MjösbergJenny MjösbergChristina StehleYanling LiuAlan M. StallAnja E. HauserYousuke TakahamaMark C. DessingGergely ToldiKlaus WarnatzRaghavendra PalankarSussan NoursharghEnrico LugliBimba F. HoyerPleun HombrinkBartek RajwaSarah WarthIsabel PanseRachael C. WalkerSilvia PiconeseAndrew FilbyPärt PetersonKilian SchoberSilvia Della BellaLeonie WegenerMerle SteinAnne CookeAlessandro MorettaDeborah KienhöferAndrea CossarizzaHyun-dong ChangKonrad Von VolkmannJessica P. HoustonMübeccel AkdisAndreas GrützkauTristan HollandJakob ZimmermannJonni S. MooreDirk MielenzIain B. McinnesBo HuangBo HuangPaulo VieiraThomas KroneisTobit SteinmetzKerstin JuelkeSharon SandersonJames V. WatsonSrijit KhanSally A. QuataertWinfried F. PicklAnnika WiedemannSara De BiasiAndreas RadbruchJames B. WingJames B. WingSusann MüllerTon N. SchumacherKaty RezvaniGloria MartrusAlexander ScheffoldToralf KaiserCarlo PucilloLara GibelliniAnna RubartelliQingyu ChengLuca BattistiniDavid MirrerDavid W. GalbraithGiovanna BorsellinoRyan R. BrinkmanRyan R. BrinkmanTim R. MosmannLaura G. RicoAnita DreherDésirée KunkelFrancesco AnnunziatoPia KvistborgAndrea GoriChiara RomagnaniAnat ShemerToshinori NakayamaFrancisco Sala-de-oyangurenAttila TárnokAlfonso BlancoAnna IannoneGiuseppe MatareseThomas DörnerVirginia LitwinMichael LohoffPetra BacherJordi PetrizLorenzo MorettaGötz R. A. EhrhardtQianjun ZhangAndrea CavaniBarry MoranChristian MaueröderImmanuel AndräDirk H. BuschJoe TrotterTimothy R D J RadstakeStipan JonjićFritz MelchersHans-martin JäckBeatriz JávegaGerald WillimskyMartin BüscherHenrik E. MeiChristine S. FalkZhigang TianZhigang TianMartin HerrmannAlice YueSteffen JungBart EvertsFrank A. SchildbergJohn Bellamy FosterGiovanna LombardiMilena NasiJohn P. NolanTodd A. FehnigerFrancesco DieliSteffen SchmittAndreas DolfA. Graham PockleyClaudia BerekJosef SpidlenMegan K. LevingsWerner MüllerBaerbel KellerRené A. W. Van LierDaisy PhilipsSusanne ZieglerChristian KurtsMalgorzata J. PodolskaJürgen RulandJürgen RulandDavid VoehringerKenneth M. MurphyMarlous Van Der BraberMaria Dolores García-godoySabine BaumgartYi ZhaoAntonio CosmaFalk HiepeCharlotte EsserPablo EngelMarcello VeldhoenIrmgard FörsterAmy E. Lovett-rackeGünnur DenizBurkhard LudewigEsther SchimiskyCristiano ScottàMarcello PintiJonathan RebhahnRegina StarkMario ClericiLiping YuShimon SakaguchiShimon SakaguchiDerek DaviesAnna Katharina SimonLorenzo CosmiGabriele MulthoffKamran GhoreschiQuirin HammerHenning UlrichJ. Paul RobinsonYvonne SamstagOlivier LantzHannes StockingerXuetao CaoXuetao CaoXuetao CaoSimon FillatreauSimon FillatreauSimon FillatreauDavid L. HavilandNatalio GarbiC. NeudörflKingston H. G. MillsSalvador Vento-asturiasChristian PethPhilip E. BoulaisDiether J. RecktenwaldBurkhard BecherTomas KalinaMichael D. LeipoldChristoph GoettlingerGemma A. FouldsJane L. GroganAxel R. SchulzJames P. Di SantoMatthias SchiemannMichael D. WardBritta EngelhardtBirgit SawitzkiAnnette OxeniusCarl S. GoodyearSalomé Leibundgut-landmannWolfgang BeiskerSue ChowCarsten WatzlMarie FolloErik LubbertsPeter WurstThomas SchülerAndreas DiefenbachWolfgang BauerHans-dieter VolkLuis E. MuñozElmar EndlGenny Del ZottoJosé-enrique O'connorMairi McgrathPaul S. Frenettesubject
0301 basic medicineT-LymphocytesCell SeparationT cell precursors0302 clinical medicineImmunophenotypingHuman lymphopoiesis[ SDV.IMM ] Life Sciences [q-bio]/ImmunologyImmunology and AllergyNon-U.S. Gov'tImmunologic Techniquemedicine.diagnostic_testResearch Support Non-U.S. Gov'tvirus diseaseshemic and immune systemsFalse Positive ReactionCell sortingFlow Cytometrynatural killer and innate lymphoid cells differentiation3. Good healthResearch Design[SDV.IMM]Life Sciences [q-bio]/ImmunologyHumanQuality Controlmedicine.drug_classImmunologyAnimals; Cell Proliferation; Cell Separation; DNA; False Positive Reactions; Flow Cytometry; Humans; Immunophenotyping; Quality Control; RNA; Research Design; Software; T-Lymphocytes; Guidelines as Topic; Immunologic Techniques; Immunology and Allergy; Immunologychemical and pharmacologic phenomenaGuidelines as TopicComputational biologyBiologyMonoclonal antibodyResearch SupportArticleFlow cytometryImmunophenotypingN.I.H.03 medical and health sciencesImmune systemImmunologic TechniqueResearch Support N.I.H. Extramuralmedicineearly lymphoid progenitorsJournal ArticleAnimalsHumansMass cytometryFalse Positive ReactionsImmunology and Allergy; Immunology; Flow cytometryIMUNOLOGIACell ProliferationAnimalExtramuralB cell ontogenyDNA030104 developmental biologyT-LymphocyteImmunologic TechniquesRNACytometrySoftware030215 immunologydescription
The marriage between immunology and cytometry is one of the most stable and productive in the recent history of science. A rapid search in PubMed shows that, as of July 2017, using “flow cytometry immunology” as a search term yields more than 68 000 articles, the first of which, interestingly, is not about lymphocytes. It might be stated that, after a short engagement, the exchange of the wedding rings between immunology and cytometry officially occurred when the idea to link fluorochromes to monoclonal antibodies came about. After this, recognizing different types of cells became relatively easy and feasible not only by using a simple fluorescence microscope, but also by a complex and sometimes esoteric instrument, the flow cytometer that is able to count hundreds of cells in a single second, and can provide repetitive results in a tireless manner. Given this, the possibility to analyse immune phenotypes in a variety of clinical conditions has changed the use of the flow cytometer, which was incidentally invented in the late 1960s to measure cellular DNA by using intercalating dyes, such as ethidium bromide. The epidemics of HIV/AIDS in the 1980s then gave a dramatic impulse to the technology of counting specific cells, since it became clear that the quantification of the number of peripheral blood CD4+ T cells was crucial to follow the course of the infection, and eventually for monitoring the therapy. As a consequence, the development of flow cytometers that had to be easy-to-use in all clinical laboratories helped to widely disseminate this technology. Nowadays, it is rare to find an immunological paper or read a conference abstract in which the authors did not use flow cytometry as the main tool to dissect the immune system and identify its fine and complex functions. Of note, recent developments have created the sophisticated technology of mass cytometry, which is able to simultaneously identify dozens of molecules at the single cell level and allows us to better understand the complexity and beauty of the immune system.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-10-01 | European Journal of Immunology |