0000000000601556

AUTHOR

Joanne Lannigan

showing 2 related works from this author

Minimal information for studies of extracellular vesicles 2018 (MISEV2018):a position statement of the International Society for Extracellular Vesicl…

2018

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines fo…

ectosomeectosomes; exosomes; extracellular vesicles; guidelines; microparticles; microvesicles; minimal information requirements; reproducibility; rigor; standardization; Histology; Cell Biology[SDV]Life Sciences [q-bio]minimal information requirementsectosomes; exosomes; extracellular vesicles; guidelines; microparticles; microvesicles; minimal information requirements; reproducibility; rigor; standardizationsize-exclusionectosomesMedicine and Health SciencesCELL-DERIVED MICROPARTICLESFIELD-FLOW FRACTIONATIONguidelinesrequirementscirculatingComputingMilieux_MISCELLANEOUSmicroparticlesManchester Cancer Research Centrelcsh:Cytologyextracellular vesicles; exosomes; ectosomes; microvesicles; minimal information requirements; guidelines; standardization; microparticles; rigor; reproducibilityPROSTATE-CANCERmicroparticleCell interactionmicrovesiclechromatographyPosition Paperextracellular vesiclesguidelineLife Sciences & Biomedicinemicrovesiclesectosomes exosomes extracellular vesicles guidelines microparticles microvesicles minimal information requirements reproducibility rigor standardizationMEMBRANE-VESICLESHistologyFETAL BOVINEEctosomes ; Exosomes ; Extracellular Vesicles ; Guidelines ; Microparticles ; Microvesicles ; Minimal Information Requirements ; Reproducibility ; Rigor ; StandardizationCIRCULATING MICROPARTICLES[SDV.BC]Life Sciences [q-bio]/Cellular Biologyexosomesddc:570exosomeSURFACE-PLASMON RESONANCEddc:610lcsh:QH573-671BiologyreproducibilitystandardizationInteracció cel·lularScience & TechnologyResearchInstitutes_Networks_Beacons/mcrcCell BiologyrigorCell membranesHUMAN URINARY EXOSOMESPREANALYTICAL PARAMETERSminimal information requirementSIZE-EXCLUSION CHROMATOGRAPHY1182 Biochemistry cell and molecular biologyextracellular vesicleHuman medicineMembranes cel·lulars
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Guidelines for the use of flow cytometry and cell sorting in immunological studies

2017

The marriage between immunology and cytometry is one of the most stable and productive in the recent history of science. A rapid search in PubMed shows that, as of July 2017, using “flow cytometry immunology” as a search term yields more than 68 000 articles, the first of which, interestingly, is not about lymphocytes. It might be stated that, after a short engagement, the exchange of the wedding rings between immunology and cytometry officially occurred when the idea to link fluorochromes to monoclonal antibodies came about. After this, recognizing different types of cells became relatively easy and feasible not only by using a simple fluorescence microscope, but also by a complex and some…

0301 basic medicineT-LymphocytesCell SeparationT cell precursors0302 clinical medicineImmunophenotypingHuman lymphopoiesis[ SDV.IMM ] Life Sciences [q-bio]/ImmunologyImmunology and AllergyNon-U.S. Gov'tImmunologic Techniquemedicine.diagnostic_testResearch Support Non-U.S. Gov'tvirus diseaseshemic and immune systemsFalse Positive ReactionCell sortingFlow Cytometrynatural killer and innate lymphoid cells differentiation3. Good healthResearch Design[SDV.IMM]Life Sciences [q-bio]/ImmunologyHumanQuality Controlmedicine.drug_classImmunologyAnimals; Cell Proliferation; Cell Separation; DNA; False Positive Reactions; Flow Cytometry; Humans; Immunophenotyping; Quality Control; RNA; Research Design; Software; T-Lymphocytes; Guidelines as Topic; Immunologic Techniques; Immunology and Allergy; Immunologychemical and pharmacologic phenomenaGuidelines as TopicComputational biologyBiologyMonoclonal antibodyResearch SupportArticleFlow cytometryImmunophenotypingN.I.H.03 medical and health sciencesImmune systemImmunologic TechniqueResearch Support N.I.H. Extramuralmedicineearly lymphoid progenitorsJournal ArticleAnimalsHumansMass cytometryFalse Positive ReactionsImmunology and Allergy; Immunology; Flow cytometryIMUNOLOGIACell ProliferationAnimalExtramuralB cell ontogenyDNA030104 developmental biologyT-LymphocyteImmunologic TechniquesRNACytometrySoftware030215 immunologyEuropean Journal of Immunology
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