Author: Ance Roga

0000000000381763

AUTHOR

Ance Roga

showing 2 related works from this author

Melanokortīna otrā tipa receptora un tā palīgproteīna ekspresija rauga Pichia Pastoris ekspresijas sistēmā

2015

Darba ietvaros tika izveidots pPIC9K- MC2R-T4L ekspresijas konstrukts, kas tika ekspresēts P.Pastoris sistēmā. Western blot analīze uzrādīja nepieciešamās molekulmasas zonu. MC2R-T4L izdevās attīrīt uz His- un Ha-tag afinitātes kolonnām. Lai noteiktu funkcionāla MC2R struktūru, to nepiecieciešams ekspresēt kompleksā ar MRAP, tādēļ tika izveidoti vairāki MC2-MRAP konstrukti, ko tālāk izmantot proteīna ekspresijai P. Pastoris šūnās. To aktivitāte tika pārbaudīta zīdītāju šūnu līnijās nosakot cAMP daudzuma izmaiņas pēc stimulācijas ar ACTH (1-24). Iegūtie rezultāti bija nestabili, tādēļ tālākajos pētījumos tiks veikta radioaktīvā ACTH saistīšanas mērījumus. Darbs tika veikts no 2013.gada jūnij…

proteīnsekspresijas sistēmaMC2RBioloģijaMRAPPichia Pastoris

researchProduct

Melanokortīnu receptoru palīgproteīna ietekme uz melanokortīnu receptoru funkcionālo aktivāciju pēc stimulācijas ar adrenokortikotropo hormonu

2013

Melanokortīnu receptora palīgproteīns (MRAP) ir nepieciešams, lai veidotos funkcionāls MC2R, taču tā ietekme uz pārējo melanokortīnu receptoru (MCR) spēju saistīt dažādus melanokortīnus vēl nav pilnībā izpētīta. Šī darba mērķis ir noteikt MRAPβ koekspresijas ietekmi uz MCR funkcionālo aktivitāti, stimulējot tos ar ACTH un α-MSH. Lai to sasniegtu tika veikta visu MCR ekspresija ar un bez MRAPβ cilvēka HEK293 šūnu līnijā, kam sekoja šūnu saražotā cAMP daudzuma izmaiņu noteikšana pēc to stimulācijas ar α-MSH un ACTH (1-24). Rezultāti pierāda, ka koekspressija ar MRAPβ palielina MCR funkcionālo atbildi pēc stimulācijas ar ACTH, savukārt pazemina - pēc stimulācijas ar α-MSH, kas norāda, ka MRAPβ…

researchProduct

PEPhysical Sciences and Engineering
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LSLife Sciences
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