Melanokortīna otrā tipa receptora un tā palīgproteīna ekspresija rauga Pichia Pastoris ekspresijas sistēmā

6533b7dbfe1ef96bd1270e92

RESEARCH PRODUCT

Melanokortīna otrā tipa receptora un tā palīgproteīna ekspresija rauga Pichia Pastoris ekspresijas sistēmā

subject

proteīns ekspresijas sistēma MC2R Bioloģija MRAP Pichia Pastoris

description

Darba ietvaros tika izveidots pPIC9K- MC2R-T4L ekspresijas konstrukts, kas tika ekspresēts P.Pastoris sistēmā. Western blot analīze uzrādīja nepieciešamās molekulmasas zonu. MC2R-T4L izdevās attīrīt uz His- un Ha-tag afinitātes kolonnām. Lai noteiktu funkcionāla MC2R struktūru, to nepiecieciešams ekspresēt kompleksā ar MRAP, tādēļ tika izveidoti vairāki MC2-MRAP konstrukti, ko tālāk izmantot proteīna ekspresijai P. Pastoris šūnās. To aktivitāte tika pārbaudīta zīdītāju šūnu līnijās nosakot cAMP daudzuma izmaiņas pēc stimulācijas ar ACTH (1-24). Iegūtie rezultāti bija nestabili, tādēļ tālākajos pētījumos tiks veikta radioaktīvā ACTH saistīšanas mērījumus. Darbs tika veikts no 2013.gada jūnija līdz 2015. gada jūnijam Latvijas Biomedicīnas pētījumu un studiju centrā.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/29476

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