0000000000382484
AUTHOR
Sally Koegel
La microdissection laser: une technique d'analyse du profil d'expression génique et protéique des types cellulaires spécifiques de la symbiose mycorhizienne à arbuscules
Expression of Sorghum bicolor ammonium transporters upon colonization with arbuscular mycorrhizal fungi
Arbuscular mycorrhizal fungi (AMF) are important plant symbionts, trading mineral nutrients beyond the reach of roots, in particular ammonium, in exchange to their host’s photosynthetic products. Sorghum bicolor is one of the world's leading cereal crops, providing food, fibre and fuel across a range of environments and production systems. It has a particular ability to be productive even under strongly adverse conditions, tolerating much more severe drought than most other grain crops. As its genome has recently been sequenced, we have characterized all eight members of the ammonium transporter (AMT) family and studied their expression in different tissues of field-grown plants. While most…
La microdissection laser: une technique d’analyse du profil d’expression génique et protéique des types cellulaires spécifiques de la symbiose mycorhizienne à arbuscules
The Largest Subunit of RNA Polymerase II as a New Marker Gene to Study Assemblages of Arbuscular Mycorrhizal Fungi in the Field
Due to the potential of arbuscular mycorrhizal fungi (AMF, Glomeromycota) to improve plant growth and soil quality, the influence of agricultural practice on their diversity continues to be an important research question. Up to now studies of community diversity in AMF have exclusively been based on nuclear ribosomal gene regions, which in AMF show high intra-organism polymorphism, seriously complicating interpretation of these data. We designed specific PCR primers for 454 sequencing of a region of the largest subunit of RNA polymerase II gene, and established a new reference dataset comprising all major AMF lineages. This gene is known to be monomorphic within fungal isolates but shows an…