0000000000382864
AUTHOR
M Mauden
Freie und glucuronidierte Cannabinoide im Urin - Untersuchungen zur Einschätzung des Konsumverhaltens
Spontanurinproben (n = 135) von ¶49 Cannabiskonsumenten wurden auf freie und glucuronidierte Tetrahydrocannabinolcarbonsaure (THCCOOH) mittels LC/MS/MS sowie auf freies und glucuronidiertes Tetrahydrocannabinol (THC) und 11-Hydroxytetrahydrocannabinol (11-OH-THC) mittels GC/MS direkt sowie nach enzymatischer Hydrolyse bis zu 10 Tagen nach dem letztmaligen Konsum untersucht. Die Einteilung des Konsumverhaltens erfolgte in schwer, moderat und leicht und orientierte sich an den Zuordnungskriterien neuerer, einschlagiger Publikationen. Die Konzentrationsangaben fur freie und glucuronidierte THCCOOH wurden auf 100 mg Kreatinin/dL Urin bezogen. In den Konsumentengruppen schwer, moderat und leicht…
Partition coefficient, blood to plasma ratio, protein binding and short-term stability of 11-nor-Delta(9)-carboxy tetrahydrocannabinol glucuronide.
11-Nor-Delta(9)-carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma ratio b/p for THCCOOglu concentrations of 100 and 500ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Delta(9)-carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in pl…
Stability of Cannabinoids in Hair Samples Exposed to Sunlight
It has been well recognized that hashish and marihuana lose potency during storage because of a decrease in the content of tetrahydrocannabinol (THC), which is the major psychoactive constituent of cannabis (1). The effect of oxygen on stored plant and resin materials or solutions of pure cannabinoids seems much less significant than that of higher temperatures (≥37 °C) or light (2)(3)(4)(5)(6)(7). A few data are available on the stability of THC and major metabolites in blood (8)(9)(10)(11). However, the stability of cannabinoids in the hair shaft has not been addressed, although scalp hair represents one of the most exposed parts of the body. Therefore, a study was performed to elucidate …