0000000000385784

AUTHOR

Jacques Magdalou

showing 3 related works from this author

Rat olfactory bulb and epithelium UDP-glucuronosyltransferase 2A1 (UGT2A1) expression: in situ mRNA localization and quantitative analysis.

2001

UDP-glucuronosyltransferases (UGTs) form a multigenic family of enzymes involved in the biotransformation and elimination of numerous endo- and xenobiotic compounds. Beside the diverse UGT isoforms present in the liver as well as in other tissues, the UGT2A1 isoform, also called olfactory UGT, was initially thought to be expressed in the nasal epithelium only. In this work, we demonstrate the UGT2A1 mRNA expression in the olfactory bulb, using in situ hybridization and quantitative reverse transcription-polymerase chain reaction (RT-PCR) techniques. Within the epithelium, UGT2A1 mRNA is mainly found in the sustentacular cells and to a lesser extent in Bowman's gland cells. Moreover, in situ…

Olfactory systemMaleCentral nervous systemNerve Tissue ProteinsIn situ hybridizationBiologyCellular and Molecular NeuroscienceMiceRapid amplification of cDNA endsOlfactory MucosaGene expressionmedicineAnimalsNeurons AfferentRNA MessengerGlucuronosyltransferaseRats WistarMolecular BiologyIn Situ HybridizationAir PollutantsMice Inbred BALB CSequence Homology Amino AcidReverse Transcriptase Polymerase Chain ReactionEpithelial CellsMolecular biologyOlfactory BulbEpitheliumOlfactory bulbRatsIsoenzymesmedicine.anatomical_structureInactivation MetabolicOlfactory epitheliumBrain research. Molecular brain research
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Carboxyl nonsteroidal anti-inflammatory drugs are efficiently glucuronidated by microsomes of the human gastrointestinal tract.

2004

Limited studies have been carried out on the biotransformation of carboxyl nonsteroidal anti-inflammatory drugs (NSAIDs) in the liver. However, the role of the intestine in NSAID metabolism has not been investigated. In this report, the contribution of UDP-glucuronosyltransferases (UGTs) in the human gastrointestinal (GI) tract from five donors to the glucuronidation of the NSAIDs, RS-ketoprofen, S-naproxen, RS- and S-etodolac, was investigated. UGT activity and, for some donors, mRNA levels were evaluated. All NSAIDs were glucuronidated throughout the GI tract; however, glucuronidation was low in stomach and duodenum as compared to the remainder of the intestine. RT-PCR analysis demonstrat…

medicine.medical_specialtyBiophysicsGlucuronidationAdministration OralPharmacologydigestive systemBiochemistryGene Expression Regulation EnzymologicFirst pass effectGlucuronidesNaproxenInternal medicineMicrosomesmedicineHumansRNA MessengerGlucuronosyltransferaseMolecular BiologyChromatography High Pressure LiquidGastrointestinal tractChemistryReverse Transcriptase Polymerase Chain ReactionStomachHuman gastrointestinal tractAnti-Inflammatory Agents Non-SteroidalUGT2B7Gastrointestinal Tractmedicine.anatomical_structureEndocrinologyKetoprofenDuodenumMicrosomeEtodolacBiochimica et biophysica acta
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Redox state alteration modulates astrocyte glucuronidation.

2004

We have investigated the effects of mild oxidative conditions on drug-metabolizing enzyme activity in rat cultured astrocytes. These experimental conditions promoting an oxidative environment were obtained by short exposure to a low concentration of menadione (5 microM) for a short duration (15 min). This resulted in the rapid and transient production of reactive oxygen species (+130%), associated with a decrease in GSH cellular content (-24%), and an increase in total protein oxidation (+26%), but promoted neither PGE(2) nor NO production. This treatment induced a rapid and persistent decrease in astrocyte glucuronidation activities, which was totally prevented by N-acetyl-l-cysteine. Thes…

MaleCell SurvivalGlucuronidationApoptosisGlucuronatesOxidative phosphorylationmedicine.disease_causeProtein oxidationBiochemistryRedoxchemistry.chemical_compoundMenadionePhysiology (medical)CricetinaemedicineAnimalsProtein IsoformsRNA MessengerGlucuronosyltransferaseRats WistarPromoter Regions GeneticCells Culturedchemistry.chemical_classificationInflammationReactive oxygen speciesBase SequenceVitamin K 3GlutathioneHydrogen PeroxideMolecular biologyGlutathioneCell biologyRatschemistryAstrocytesFemaleReactive Oxygen SpeciesOxidation-ReductionOxidative stressFree radical biologymedicine
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