0000000000389019
AUTHOR
María Pelechá
showing 2 related works from this author
Improved in vivo efficacy of clinical-grade cryopreserved human hepatocytes in mice with acute liver failure.
2020
Clinical hepatocyte transplantation short-term efficacy has been demonstrated; however, some major limitations, mainly due to the shortage of organs, the lack of quality of isolated cells and the low cell engraftment after transplantation, should be solved for increasing its efficacy in clinical applications. Cellular stress during isolation causes an unpredictable loss of attachment ability of the cells, which can be aggravated by cryopreservation and thawing. In this work, we focused on the use of a Good Manufacturing Practice (GMP) solution compared with the standard cryopreservation medium, the University of Wisconsin medium, for the purpose of improving the functional quality of cells …
Induced pluripotent stem cells in liver disease
2021
Abstract The development of suitable and reproducible liver cell models is fundamental for regenerative medicine, drug screening, and disease modeling. Human primary hepatocytes are the gold standard not only for hepatic cell therapy but also for preclinical toxicological screening or the study of liver disease; however, their limited availability, variability, and phenotypic instability hamper their use. Recent advances in stem cell technology have allowed the generation of induced pluripotent stem cells (iPSCs) from different somatic cell types and their differentiation into hepatocyte-like cells (HLCs), which can provide unlimited cell source for their use in hepatology studies. We revie…