0000000000394036
AUTHOR
Hyeon-yong Park
The apparent loss of tissue culture competence during leaf differentiation in yams (Dioscorea bulbifera L.)
Explants taken from the leaves of yams (Dioscorea bulbifera L.) at different stages of development were cultured in vitro on a checkerboard using various combinations and/or concentrations of auxin (2,4-d) and cytokinin (6-BAP). An addition of cytokinin to the culture media was not essential for callus induction from explants derived from young leaves in the very early stages of expansion. When the leaves expanded further they required cytokinin and the requirement increased considerably during expansion. Explants taken from fully expanded leaves were no longer able to proliferate, even when extremely high concentrations of cytokinins were applied. Callus grown from highly immature leaves w…
Improved Mesophyll Protoplast Culture and Plant Regeneration in Arabidopsis thaliana (L.) Heynh., Genotype Landsberg Erecta
Summary The response of in vitro cultured Arabidopsis thaliana mesophyll protoplasts was investigated with the aim of characterising the crucial factors that affect plating efficiencies and plant regeneration. We selected the genotype Landsberg erecta, which is frequently used for genetic studies, but which is known to respond poorly in protoplast culture. Factors permitting vigorous vegetative growth of the donor plants, i.e. short light period and avoidance of high temperature, were found to be advantageous. Selection of competent leaves and fractionation of protoplast populations indicated that predominantly protoplasts derived from still expanding, yet not highly immature mesophyll cell…