0000000000406273
AUTHOR
Ana Isabel Doblas Expósito
Off-axis Digital Holographic Microscopy: practical design parameters for operating at diffraction limit
The utilization of microscope objectives (MOs) in digital holographic microscopy (DHM) has associated effects that are not present in conventional optical microscopy. The remaining phase curvature, which can ruin the quantitative phase imaging, is the most evident and analyzed. As phase imaging is considered, this interest has made possible the development of different methods of overcoming its undesired consequences. Additionally to the effects in phase imaging, there exist a set of less obvious conditions that have to be accounted for as MOs are utilized in DHM to achieve diffraction-limit operation. These conditions have to be considered even in the case in which only amplitude or intens…
New advances in high-resolution optical microscopy
La microscopio ía tiene como nalidad observar muestras que no pueden distinguirse a simple vista por el ojo humano dado que el tamaño de ellas es menor que su l mite de resoluci on. Un microscopio produce una imagen ampliada de la muestra a analizar. Debido a que la microscopía es un instrumento b asico para la ciencia de la vida y de los materiales, en esta Tesis se desarrolla un estudio exhaustivo de la microscopía óptica. Los microscopios opticos se pueden con gurar de diferentes formas para producir im agenes con diferentes caracter sticas. El microscopio de campo amplio, el m as simple de todos, presenta algunas limitaciones que debe ser superadas para obtener im agenes de mayor calida…
Diabetes screening by telecentric digital holographic microscopy
Diabetes is currently the world's fastest growing chronic disease and it is caused by deficient production of insulin by the endocrine pancreas or by abnormal insulin action in peripheral tissues. This results in persistent hyperglycaemia that over time may produce chronic diabetic complications. Determination of glycated haemoglobin level is currently the gold standard method to evaluate and control sustained hyperglycaemia in diabetic people. This measurement is currently made by high-performance liquid chromatography, which is a complex chemical process that requires the extraction of blood from the antecubital vein. To reduce the complexity of that measurement, we propose a fully-optica…