0000000000409041

AUTHOR

Giuseppe Arcoleo

showing 5 related works from this author

Validation of Loop-Mediated Isothermal Amplification (LAMP) Field Tool for Rapid and Sensitive Diagnosis of Contagious Agalactia in Small Ruminants

2020

Simple Summary Contagious agalactia (CA) is an infectious disease of small ruminants endemic in the Mediterranean countries, causing significant socioeconomic impacts predominantly on small-scale farmers who still subsist on marginal lands. Mycoplasma agalactiae is historically considered the principal etiological agent of CA, especially in sheep. Clinical signs are characterised by mastitis, arthritis, keratoconjunctivitis and occasionally, abortion. Rapid, accurate and cost-effective field tests are urgently needed for effective control of M. agalactiae mastitis. Our study illustrated the validation of a Loop-Mediated Isothermal Amplification (LAMP) test for the detection of M. agalactiae…

Veterinary medicinelcsh:Veterinary medicineGeneral Veterinaryved/biologyMycoplasma agalactiaeCommunicationfield diagnostic testved/biology.organism_classification_rank.species<i>mycoplasma agalactiae</i>p40 geneLoop-mediated isothermal amplificationDiagnostic testBiologyMycoplasma agalactiaeLAMPFluorometersmall ruminantlcsh:Zoology<i>p40</i> genelcsh:SF600-1100Animal Science and Zoologylcsh:QL1-991small ruminantsTarget geneAnimals : an Open Access Journal from MDPI
researchProduct

Cigarette smoke affects the onco-suppressor DAB2IP expression in bronchial epithelial cells of COPD patients

2019

AbstractCigarette smoke is a risk factor for COPD and lung cancer. In cancer, epigenetic modifications affect the expression of Enhancer of Zester Homolog 2 (EZH2), and silenced disabled homolog 2 interacting protein gene (DAB2IP) (onco-suppressor gene) by Histone H3 tri-methylation in lysine 27 (H3K27me3). In“ex vivo”studies, we assessed EZH2, H3K27me3 and DAB2IP immunoreactivity in bronchial epithelial cells from COPD patients (smokers, ex-smokers), Smoker and control subjects. In“in vitro” experiments we studied the effect of cigarette smoke extract (CSE) on EZH2/H3K27me3/DAB2IP expression, apoptosis, invasiveness, and vimentin expression in 16HBE, primary cells, and lung cancer cell lin…

Jumonji Domain-Containing Histone DemethylasesLung NeoplasmsCigar SmokingCelllcsh:MedicineApoptosismacromolecular substancesArticlePulmonary Disease Chronic ObstructiveRisk FactorsmedicineHumansEnhancer of Zeste Homolog 2 ProteinNeoplasm Invasivenesslcsh:ScienceLung cancerA549 CellOncogenesisInflammationA549 cellRegulation of gene expressionCOPDMultidisciplinarybusiness.industrylcsh:REZH2ApoptosiJumonji Domain-Containing Histone DemethylaseCancerras GTPase-Activating Proteinmedicine.diseaseAlveolar Epithelial Cellrespiratory tract diseasesLung NeoplasmGene Expression Regulation NeoplasticNeoplasm Invasiveness Pulmonary Disease Chronic Obstructivemedicine.anatomical_structureA549 Cellsras GTPase-Activating ProteinsApoptosisAlveolar Epithelial CellsCancer researchlcsh:QbusinessHumanairway disease
researchProduct

COVID-19 and non–COVID-19 pneumonia: a comparison

2021

Abstract Background The COVID-19 pandemic has caused the relocation of huge financial resources to departments dedicated to infected patients, at the expense of those suffering from other pathologies. Aim To compare clinical features and outcomes in COVID-19 pneumonia and non-COVID-19 pneumonia patients. Patients and methods 53 patients (35 males, mean age 61.5 years) with COVID-19 pneumonia and 50 patients (32 males, mean age 72.7 years) with non-COVID-19 pneumonia, consecutively admitted between March and May 2020 were included. Clinical, laboratory and radiological data at admission were analyzed. Duration of hospitalization and mortality rates were evaluated. Results Among the non-COVID…

Maleground-glass opacitiesmedicine.medical_specialtypolmonitePneumonia ViralPopulationmortalitàComorbidityDiseaseresourceelderlyInternal medicinePandemicPulmonary MedicinemedicineHumanspneumoniahealth systemeducationPandemicsDisease burdenopacità a vetro smerigliatoRetrospective StudiesAgededucation.field_of_studybusiness.industrySARS-CoV-2Mortality raterisorseCOVID-19General MedicineMiddle Agedmedicine.diseaseComorbiditymortalityPneumoniaground-glass opacitieCOVID-19 Nucleic Acid TestingHRCTricoverianzianiresourcesbusinesssistema sanitarioResearch ArticleKidney diseasehospitalization
researchProduct

Effects of aging on sensation of dyspnea and health-related quality of life in elderly asthmatics

2005

BACKGROUND AND AIMS: As the awareness of sensory stimuli is often impaired in older subjects, it has been hypothesized that the aging process may influence the perception of dyspnea. This study aimed at evaluating the aging-related difference in perception of spontaneously occurring dyspnea in adult asthmatics and at whether any such aging-related differences have an effect on the health-related quality of life (HRQOL). METHODS: 18 elderly asthmatics (EA) aged >65 years and 20 young asthmatics (YA) (age range 16-44 years) were recruited. In all subjects, 12-month asthma symptom score and respiratory function were recorded. Dyspnea was measured at rest by the Visual Analog Scale (VAS) and HR…

AdultMaleAgingmedicine.medical_specialtyAdolescentVisual analogue scaleHealth StatusStatistics as TopicSettore MED/10 - Malattie Dell'Apparato RespiratorioQuality of lifeSensationmedicineHumansRespiratory functionRespiratory systemAgedAsthmaHealth related quality of lifebusiness.industryAsthma symptomsmedicine.diseaseAsthmarespiratory tract diseasesDyspneaQuality of LifePhysical therapyFemaleGeriatrics and GerontologybusinessAging Clinical and Experimental Research
researchProduct

Cigarette smoke alters the EZH2/DAB2IP expression in bronchial epithelial cells. A risk factor for lung cancer in COPD patients

2016

Cigarette smoke is an environmental risk factor for COPD and Lung cancer, leading causes of morbidity and mortality worldwide. In cancer, enhancer of zeste homolog 2 (EZH2) silenced disabled homolog 2 interacting protein gene (DAB2IP) (tumor-suppressor gene) bytri-methylation of lysine 27 on histone H3 (H3K27me3). We studied EZH2 and DAB2IP expression in airway epithelial cells from COPD patients and their potential involvement in the progression of COPD toward lung cancer. We assessed EZH2 and DAB2IPimmunoreactivity in bronchial epithelial cells from surgical specimens of COPD patients and healthy control subjects (HC) by immunohistochemistry. Bronchial epithelial cell line (16HBE), primar…

COPDLungbusiness.industryEZH2Cancermacromolecular substancesrespiratory systemmedicine.diseaserespiratory tract diseasesHistone H3medicine.anatomical_structureApoptosisImmunologymedicineCancer researchImmunohistochemistrybusinessLung cancer3.2 Airway Cell Biology and Immunopathology
researchProduct