0000000000409270
AUTHOR
H. Rieger
Influence of therapeutic and toxic doses of neuroleptics and antidepressants on energy metabolism of the isolated perfused rat brain.
The isolated perfused rat brain was used for a comparative study of the effects of promazine, imipramine, monodesmethyl promazine and desipramine on cerebral energy metabolism. After perfusion for 30 min or 1 h the brain levels of the following substrates and metabolites were estimated: P-creatine, creatine, ATP, ADP, AMP, glycogen, glucose, glucose-6-P, fructose diphosphate, dihydroxyacetone-P, pyruvate, lactate, α-ketoglutarate, and ammonia. Drug concentrations of 5·10−6 M and 10−5 M in the perfusion medium caused a significant decrease of glucose-6-P alone. When the drug concentration was raised to a toxic range (10−4 M), reflected in the EEG by the pattern of secondary discharges, an ac…
Is the EOG Influenced by Nicotine? Relations to the EEG
Cigarette smoking causes a number of effects in the human body, not the least of which may be attributed to nicotine. The problem under investigation is whether, by smoking retinal activity is influenced, and whether measureable changes are found by electrophysiological testing.
Microrheology of erythrocytes and platelets: physiological basis and consequences for the design and the operation of extracorporeal circulatory devices
Publisher Summary This chapter discusses the physiological basis and consequences for the design and the operation of extracorporeal circulatory devices. The microrheological response of erythrocytes and thrombocytes can be observed microscopically under simplified flow conditions in the rheoscope. In this device, cells are observed at high magnifications while being subjected to quantifiable shear stresses. Despite the unphysiological environment, the use of this method allowed an important extrapolation to well-established in vivo flow properties and a clear distinction between the primarily passive microrheological properties of the erythrocytes and the microrheological features of throm…
Comparison of the effects of chloral hydrate and trichlorethanol on the EEG of the isolated perfused rat brain.
An isolated perfused rat brain preparation was used to compare the effects of chloral hydrate and its metabolite trichloroethanol on the EEG. The concentrations of chloral hydrate and trichloroethanol in the perfusion medium ranged from 1.5 to 5.5 mM. 5, 10, 15, and 30 min after the beginning of the perfusions EEG-recordings were taken. The recordings were evaluated both by a descriptive method and by a simple quantitative appraoch, counting the waves with an amplitude greater than 50 microvolts and averaging this value for a period of 1 sec. The following results were obtained: Both drugs exhibited CNS depressant activity. Between 5 and 10 min of perfusion the effect of trichloroethanol wa…
Quantitative analysis of the EEG effects produced by imipramine, desipramine, promazine, and monodesmethyl promazine in the isolated perfused rat brain
The effects of imipramine, desipramine, promazine and monodesmethyl promazine on the EEG of the isolated perfused rat brain were studied. The brain preparation was perfused for 30 min with simulated blood, containing of the drugs in a concentration of 10−5 M. Control experiments were performed without a drug added to the simplified blood. The EEG was recorded at various times on a magnetic tape and was evaluated visually and quantitatively (amplitude and interval histography). The EEG effects of imipramine and promazine as well as the effects of these drugs with their monodesmethyl metabolites were compared. The drugs produced clear EEG changes compared with the control EEG. An increase of …