Novel biomarker panel for colon cancer unveiled by proteomics

Proteomic analysis of exosome-like vesicles derived from breast cancer cells.

Background/Aim: The phenomenon of membrane vesicle-release by neoplastic cells is a growing field of interest in cancer research, due to their potential role in carrying a large array of tumor antigens when secreted into the extracellular medium. In particular, experimental evidence show that at least some of the tumor markers detected in the blood circulation of mammary carcinoma patients are carried by membrane-bound vesicles. Thus, biomarker research in breast cancer can gain great benefits from vesicle characterization. Materials and Methods: Conditioned medium was collected from serum starved MDA-MB-231 sub-confluent cell cultures and exosome-like vesicles (ELVs) were isolated by ultra…

Potentially useful diagnostic and prognostic markers in colon cancer: a proteomic-based investigation

Functional investigations of matrix metalloproteinases in breast cancer: focus on MMP9 and MMP2