0000000000416182
AUTHOR
Dierk Ratnatunga
N transport in the Hebeloma cylindrosporum – Pinus pinaster ectomycorrhizal association
International audience; Studies of nitrogen metabolism in ectomycorrhizas have demonstrated that the fungal symbiont plays a fully integrated role in plant root metabolism and participates actively in the assimilation and transfer of newly absorbed nitrogen compounds by transferring amino acids. In symbiotic associations, such as mycorrhizas, location of the symbiosis ensures that mycelial growth of the fungus into soil provides access to nutrients of the soil solution. Changes in the distribution and activity of membrane-bound transport proteins in response to symbiotic interactions need to be investigated. In recent years several genes from H. cylindrosporum and P. pinaster, putatively in…
RNAi assays in the model fungus Hebeloma cylindrosporum
International audience; Posttranscriptional gene silencing is a mechanism of suppressing gene expression in a sequence specific manner. This naturally occurring phenomenon of RNA interference (RNAi) has been used by researchers for gene function analysis in plants, fungi and animals. There is currently no report on RNAi establishment in mycorrhizal fungi. In order to obtain an insight into the in-vivo function of recently described genes coding for transporters and enzymes involved in N uptake and metabolism (Müller et al., 2007) from H. cylindrosporum, it is necessary to establish several methods for genetic analysis. After successfully expressing an EGFP in the mycelium of H.c. (Müller et…
Isolation and characterization of N transporters putatively involved in the Hebeloma cylindrosporum-Pinus pinaster ectomycorrhizal association
International audience; Studies of nitrogen metabolism in ectomycorrhizas have demonstrated that the fungal symbiont plays a fully integrated role in plant root metabolism and participates actively in the assimilation and transfer of newly absorbed nitrogen compounds by transferring amino acids. In symbiotic associations, such as mycorrhizas, location of the symbiosis ensures that mycelial growth of the fungus into soil provides access to nutrients in the soil solution. Changes in the distribution and activity of membrane-bound transport proteins in response to symbiotic interactions need to be investigated. In recent years several genes from H. cylindrosporum and P. pinaster, putatively in…