0000000000416613

AUTHOR

Juris Steinbergs

showing 3 related works from this author

cDNA Synthesis and Cloning

1998

The isolation of intact messenger RNA and its conversion into cDNA copies by avian or Moloney murine reverse transcriptase, as well as subsequent amplification of gene transcripts by the PCR technique, are becoming increasingly important tools in molecular biology. At present, these techniques have been often necessary and widely used for the analysis of individual mRNA levels in cells and tissues by Northern blot analysis, nuclease protection analysis and in situ hybridization. Another important application of RNA templates is the construction of representa­tive cDNA libraries in order to clone genes, to investigate their molecular structure and to express them in prokaryotic and/or eukary…

CloningMessenger RNAcDNA libraryComplementary DNARNANorthern blotBiologyMolecular biologyGeneReverse transcriptase
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Molecular Basis for the Interaction of the Hepatitis B Virus Core Antigen with the Surface Immunoglobulin Receptor on Naive B Cells

2001

ABSTRACTThe nucleocapsid of the hepatitis B virus (HBV) is composed of 180 to 240 copies of the HBV core (HBc) protein. HBc antigen (HBcAg) capsids are extremely immunogenic and can activate naive B cells by cross-linking their surface receptors. The molecular basis for the interaction between HBcAg and naive B cells is not known. The functionality of this activation was evidenced in that low concentrations of HBcAg, but not the nonparticulate homologue HBV envelope antigen (HBeAg), could prime naive B cells to produce anti-HBc in vitro with splenocytes from HBcAg- and HBeAg-specific T-cell receptor transgenic mice. The frequency of these HBcAg-binding B cells was estimated by both hybridom…

CD4-Positive T-LymphocytesImmunologyNaive B cellAntigen presentationMolecular Sequence DataImmunoglobulin Variable RegionMice Transgenicmedicine.disease_causeAntibodies ViralMicrobiologyMiceAntigenVirologymedicineAnimalsHumansAmino Acid SequenceReceptors ImmunologicHepatitis B virusAntigen PresentationB-LymphocytesMice Inbred BALB Cbiologyvirus diseasesAntibodies MonoclonalVirologyMolecular biologyHepatitis B Core Antigensdigestive system diseasesPeptide FragmentsVirus-Cell InteractionsHBcAgHBeAgImmunoglobulin MImmunoglobulin MInsect Sciencebiology.proteinMice Inbred CBAImmunoglobulin Light ChainsBinding Sites AntibodyAntibodyImmunoglobulin Heavy ChainsSequence Alignment
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Short synthetic CDR-peptides forming the antibody combining site of the monoclonal antibody against RNA bacteriophage fr neutralize the phage activit…

1996

The construction of a mouse hybridoma FRS2 secreting neutralizing monoclonal antibody specific for RNA bacteriophages fr, MS2 and GA is reported. The genes encoding the variable domains of the monoclonal antibody FRS2 heavy and light chains were cloned and sequenced and the corresponding complementarity determining region (CDR) peptides were chemically synthesized. The CDR-peptides were tested for their ability to neutralize the activity of RNA phage fr and related RNA phages MS2 and GA. The CDR-derived peptides H2, L2 and L3 interacted with the fr phage particles and neutralized fr phage activity. Two of these peptides-H2 and L3 also had the ability to neutralize partly the activity of rel…

medicine.drug_classvirusesImmunologyMolecular Sequence DataImmunoglobulin Variable Regionchemical and pharmacologic phenomenaEnzyme-Linked Immunosorbent AssayComplementarity determining regionRNA PhagesMonoclonal antibodyBacteriophageMiceAntigenNeutralization TestsBacteriophage MS2medicineImmunology and AllergyAnimalsAmino Acid SequenceCloning MolecularMicroscopy ImmunoelectronMice Inbred BALB CbiologyBase SequenceRNAAntibodies MonoclonalGeneral MedicineRNA Phagesbiology.organism_classificationMolecular biologyPeptide Fragmentsbiology.proteinAntibodyHuman antibodies and hybridomas
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