In vitro establishment and culture of two Sicilian cultivars of Olea europaea L

Sicily is among the Italian regions with the largest cultivation area of olive (Olea europaea L.). The applications of in vitro tissue culture, as practical tool of propagation and germplasm storage, were not sufficiently exploited in this species, because of the lack of efficient in vitro establishment protocols and culture for the majority of the cultivars. In fact, it is well known that the success of in vitro culture is strongly dependent on the species and, within the species, on the cultivars. This study reports the in vitro establishment of two Sicilian olive cultivars, grown in greenhouse. Both genotypes showed a percentage of in vitro sprouting higher than that one previously obtai…

IN VITRO GERMINATION AND SEEDLING DEVELOPMENT OF CAPER (CAPPARIS SPINOSA L.)

IN VITRO GERMINATION OF STONELESS AND ISOLATED EMBRYOS OF 14 SICILIAN CULTIVARS OF OLEA EUROPAEA L.

Microspore Culture in eleven Cultivars of Loquat

In plant breeding, microspore embryogenesis via isolated microspore culture is increasing as a method to obtain in a single-step doubled haploids and homozygosity. In this study, isolated microspore culture of eleven cultivars of loquat (Eriobotrya japonica (Thunb.) Lindl.) has been carried out. An analysis of anthers from different flower bud sizes, has been carried out, to identify and to select the flower bud size with anthers containing the highest quantity of vacuolated microspores. After a pre-treatment of the panicles at 4°C in dark for 4 days, flower buds were sterilized and microspores were isolated and cultured in two different media (P and MB). Moreover, only the isolated microsp…