0000000000427710

AUTHOR

Rene Tolba

showing 2 related works from this author

Fetuin-B, a liver-derived plasma protein is essential for fertilization.

2013

SummaryThe zona pellucida (ZP) is a glycoprotein matrix surrounding mammalian oocytes. Upon fertilization, ZP hardening prevents sperm from binding to and penetrating the ZP. Here, we report that targeted gene deletion of the liver-derived plasma protein fetuin-B causes premature ZP hardening and, consequently, female infertility. Transplanting fetuin-B-deficient ovaries into wild-type recipients restores fertility, indicating that plasma fetuin-B is necessary and sufficient for fertilization. In vitro fertilization of oocytes from fetuin-B-deficient mice only worked after rendering the ZP penetrable by laser perforation. Mechanistically, fetuin-B sustains fertility by inhibiting ovastacin,…

Malemedicine.medical_specialtyCell Membrane Permeabilitymedicine.medical_treatmentmacromolecular substancesFertilization in VitroBiologyGeneral Biochemistry Genetics and Molecular BiologyMiceHuman fertilizationPregnancyInternal medicinemedicineAnimalsZona pellucidaMolecular BiologyZona Pellucidachemistry.chemical_classificationProteaseOvaryGene Expression Regulation DevelopmentalEmbryoCell BiologyEmbryo TransferEmbryo MammalianSpermFetuinSpermatozoaFetuin-BRecombinant ProteinsCell biologyEnzyme ActivationMice Inbred C57BLmedicine.anatomical_structureEndocrinologychemistryFertilizationMetalloproteasesOocytesGameteFemaleGlycoproteinInfertility FemaleDevelopmental BiologyDevelopmental cell
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Design of composite measure schemes for comparative severity assessment in animal-based neuroscience research: A case study focussed on rat epilepsy …

2020

PLOS ONE 15(5), e0230141 (2020). doi:10.1371/journal.pone.0230141

Computer sciencePhysiologyPsychological interventionSocial Sciencescomputer.software_genreOpen fieldField (computer science)Rats Sprague-Dawley0302 clinical medicineMathematical and Statistical TechniquesMedicine and Health SciencesPsychologyCluster Analysis0303 health sciencesPrincipal Component AnalysisMultidisciplinaryAnimal Welfare (journal)Animal BehaviorQStatisticsRAnimal ModelsResearch AssessmentNeurologyExperimental Organism SystemsAnimal SocialityPhysical SciencesMedicineDisease Models Animals epilepsy animal behaviorFemaleLocomotionResearch ArticleScienceSpatial BehaviorContext (language use)Machine learningResearch and Analysis Methods03 medical and health sciencesRobustness (computer science)Animal welfareKindling NeurologicAnimalsRelevance (information retrieval)BurrowingStatistical MethodsSocial BehaviorSelection (genetic algorithm)030304 developmental biologyBehaviorEpilepsybusiness.industryBiological LocomotionBiology and Life SciencesRatsDisease Models AnimalBiological Variation PopulationMultivariate AnalysisAnimal StudiesArtificial intelligenceK Means ClusteringbusinesscomputerZoology030217 neurology & neurosurgeryMathematicsSoftware
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