0000000000430756

AUTHOR

S. Distefano

showing 6 related works from this author

Detection of bacteria and yeast species by the BACTEC 9120 automated system with the routine use of aerobic, anaerobic, and fungal media

2008

ABSTRACT During the period 2006 and 2007, all blood cultures required by four units at high infective risk and most of those required by other units of the University Hospital of Palermo, Palermo, Italy were performed using a Bactec 9120 automated blood culture system with a complete set of Plus Aerobic/F, Plus Anaerobic/F, and Mycosis IC/F bottles. The aim of the study was to enable the authors to gain firsthand experience of the culture potentialities of the three different media, to obtain information regarding the overall and specific recovery of bacteria and yeasts from blood cultures in the hospital, and to reach a decision as to whether and when to utilize anaerobic and fungal bottle…

Microbiology (medical)BacilliSettore MED/07 - Microbiologia E Microbiologia ClinicaAutomated blood cultures Mycosis IC/F Candida albicansMycologyBiologyMicrobiologyAutomationSepsisYeastsmedicineHumansBlood cultureAnaerobiosisCandida albicansMycosisFungemiamedicine.diagnostic_testBacteriaObligate anaerobeBacterial Infectionsmedicine.diseasebiology.organism_classificationAerobiosisCulture MediaBloodItalyMycosesAnaerobic exerciseBacteria
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Identification of Candida dubliniensis among oral yeast isolates from an Italian population of human immunodeficiency virus-infected (HIV+) subjects

2002

Candida dubliniensis, an emerging oral pathogen, phenotypically resembles Candida albicans so closely that it is easily misidentified as such. The aim of the present study was to evaluate the usefulness of two phenotypic methods, growth at 45 degrees C and 2,3,5-triphenyltetrazolium chloride (TTC) reduction, for confirming presumptive identification of C. dubliniensis and C. albicans by colony color on CHROMagar Candida (CAC) medium. A combination of these methods was used to establish the prevalence of oral C. dubliniensis in an Italian population of 45 human immunodeficiency virus (HIV)-infected subjects. Twenty-two samples (48.9%) were positive for yeasts on CAC medium producing a total …

Microbiology (medical)ImmunologyFungi imperfectiBiologybiology.organism_classificationmedicine.diseaseMicrobiologyVirologyCorpus albicansVirusMicrobiologymedicinecardiovascular diseasesCandida albicansGeneral DentistryPathogenCandida dubliniensisFluconazoleMycosismedicine.drugOral Microbiology and Immunology
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Characterization of Acinetobacter baumannii from intensive care units and home care patients in Palermo, Italy.

2011

AbstractIn this study 45 isolates of Acinetobacter baumannii identified from patients in intensive care units of three different hospitals and from pressure ulcers in home care patients in Palermo, Italy, during a 3-month period in 2010, were characterized. All isolates were resistant to at least three classes of antibiotics, but susceptible to colistin and tygecycline. Forty isolates were non-susceptible to carbapenems. Eighteen and two isolates, respectively, carried the blaOXA-23-like and the blaOXA-58-like genes. One strain carried the VIM-4 gene. Six major rep-PCR subtype clusters were defined, including isolates from different hospitals or home care patients. The sequence type/pulsed …

Microbiology (medical)Acinetobacter baumanniimedicine.medical_specialtyPediatricsSettore MED/07 - Microbiologia E Microbiologia ClinicaGenotypeDrug resistanceMicrobial Sensitivity Testshome care patientsintensive care unitbeta-Lactamaseslaw.inventionlawmultidrug resistanceIntensive careInternal medicineDrug Resistance Multiple BacterialGenotypepolycyclic compoundsmedicinePulsed-field gel electrophoresisCluster AnalysisHumansCross Infectionbiologybusiness.industryGeneral Medicinebiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationIntensive care unitHome Care ServicesAcinetobacter baumanniiAnti-Bacterial AgentsElectrophoresis Gel Pulsed-FieldCommunity-Acquired InfectionsMolecular TypingIntensive Care UnitsInfectious DiseasesItalyColistinMultilocus sequence typingepidemiologybusinesshome caremedicine.drugAcinetobacter InfectionsMultilocus Sequence Typing
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Cluster of Legionnaires’ Disease in an Italian Prison

2019

Background: Legionella pneumophila (Lp) is the most common etiologic agent causing Legionnaires&rsquo

SystemVeterinary medicine<i>Legionella pneumophila</i>Health Toxicology and Mutagenesismedia_common.quotation_subjectwaterlcsh:MedicinePrison030230 surgeryReference laboratoryDisease clusterLegionella pneumophilaArticleLegionella pneumophila03 medical and health sciences0302 clinical medicinePrevalencemedicineCluster AnalysisHumansTypingclusterGenotypingmedia_common0303 health sciencesbiology030306 microbiologylcsh:RPublic Health Environmental and Occupational HealthSequence typesbacterial infections and mycosesbiology.organism_classificationmedicine.diseaserespiratory tract diseasesItalyPrisonsbacteriasystemsLegionnaires' diseaseprisonLegionnaires' DiseaseWater MicrobiologyEnvironmental MonitoringInternational Journal of Environmental Research and Public Health
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An Update of the Evolving Epidemic of blaKPC Carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of Multiple Non-ST258 Clones

2015

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute…

CarbapenemKlebsiella pneumoniaelcsh:MedicineGene ExpressionDrug resistancePlasmidbeta-LactamaseDisease OutbreaksMolecular typingFluoroquinoloneDrug Resistance Multiple Bacterialpolycyclic compoundslcsh:ScienceCarbapenemMembrane ProteinDisease OutbreakMultidisciplinarybiologyMedicine (all)IncidenceHospitalsAnti-Bacterial AgentsElectrophoresis Gel Pulsed-FieldKlebsiella pneumoniaeItalyEpidemiological MonitoringHumanFluoroquinolonesPlasmidsResearch Articlemedicine.drugBacterial ProteinAminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Clone Cells; Colistin; Drug Resistance Multiple Bacterial; Electrophoresis Gel Pulsed-Field; Epidemiological Monitoring; Fluoroquinolones; Gene Expression; Hospitals; Humans; Incidence; Italy; Klebsiella Infections; Klebsiella pneumoniae; Membrane Proteins; Multilocus Sequence Typing; Mutation; Plasmids; beta-Lactamases; Disease Outbreaks; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)beta-LactamasesMicrobiologyClone CellHospitalAntibiotic resistanceBacterial ProteinsAnti-Bacterial AgentmedicineHumansBiochemistry Genetics and Molecular Biology (all)AminoglycosideColistinlcsh:RMembrane ProteinsCarbapenemase producingbiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationVirologyClone CellsKlebsiella InfectionsAminoglycosidesAgricultural and Biological Sciences (all)CarbapenemsMutationColistinMultilocus sequence typinglcsh:QKlebsiella InfectionMultilocus Sequence TypingPLOS ONE
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Genotyping of Legionella pneumophila serogroup 1 strains isolated in Northern Sicily, Italy.

2008

During a three-year period, from April 2002 to May 2005, one hundred-forty-seven samples, taken from technical systems of water distribution at point of use, were repeatedly collected at six different sites in Northern Sicily and assayed for the presence of Legionella pneumophila serogroup 1 and serogroups 2 to 14. At the first samplings, the water distribution systems of all the sites were heavily contaminated, and disinfection treatments by the superheat and flush method were therefore performed. Treatments were always successful against L. pneumophila sg.1, but only in a few cases against all other serogroups. Eighty-six strains of L. pneumophila sg. 1, isolated from 26 of these samples,…

DNA BacterialDisinfectionMolecular EpidemiologySettore MED/07 - Microbiologia E Microbiologia ClinicaGenotypeCluster AnalysisFresh WaterSequence Analysis DNAAmplified Fragment Length Polymorphism AnalysisLegionella pneumophila Surveillance Water distribution system Molecular typing Amplified fragment length polymorphismSicilyBacterial Typing TechniquesLegionella pneumophila
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