Frequency and subset distribution of human CD8 T cells specific for mycobacterial peptides in healthy contact individuals.

Effects of the static magnetic field generated by 0.5 T MRI unit on TNF-α release of human PBMC from patients with rheumatoid arthritis.

Cross-talk between gamma delta T cells and dendritic cells in anti-mycobacterial immune response

D6 is a decoy and scavenger receptor for inflammatory CC chemokines. D6-deficient mice were rapidly killed by intranasal administration of low doses of Mycobacterium tuberculosis. The death of D6(-/-) mice was associated with a dramatic local and systemic inflammatory response with levels of M. tuberculosis colony-forming units similar to control D6-proficient mice. D6-deficient mice showed an increased numbers of mononuclear cells (macrophages, dendritic cells, and CD4 and CD8 T lymphocytes) infiltrating inflamed tissues and lymph nodes, as well as abnormal increased concentrations of CC chemokines (CCL2, CCL3, CCL4, and CCL5) and proinflammatory cytokines (tumor necrosis factor alpha, int…

ThO to Th1 switch of CD4T cell clones specific from the 16-kDa antigen of Mycobacterium tuberculosis after successfull therapy: lack of involvement of epitope repertoire and HLA-DR

Effects of the static magnetic field generated by a 0.5 MRI unit on intracellular calcium concentration and IFN- g release of human CD4 T cells

Efficacy and safety of γδT cell-based tumor immunotherapy: a meta-analysis.

Vγ9Vδ2 T cells are important effector cells that may play a role in the anti-tumor immune response. Their capability to exert MHC-nonrestricted lytic activity against different tumor cells in vitro and their detection among tumor infiltrating lymphocytes in a variety of human cancers have supported the development of Vγ9Vδ2 T cell-based immunotherapy in the context of novel treatment against cancer. Accordingly, promising reports from recent clinical trials support the use of V γ9Vδ2 T cells as immunotherapeutic agents, either via adoptive transfer of ex-vivo expanded V γ9Vδ2 T cells or in vivo activation of V γ9Vδ2 T cells with compounds such as phosphoantigens or aminobisphosphonates. In …

A SUBSET OF VGAMMA9 VDELTA2 T CELLS HELP B CELLS FOR ANTIBODY PRODUCTION.

In vitro effects of aminobisphosphonates on Vgamma9Vdelta2 T cell activation and differentiation.

Selection of microbial epitopes for immune recognition. North-South transfer in Biotechnology of Tuberculosis and AIDS. University of Rome “Tor Vergata”. UNESCO Interdisciplinary Chair in Biotechnology. Vittorio Colizzi, Luc Mantagnier, 2004

Differentiation, phenotype, and function of interleukin-17-producing human V{gamma}9V{delta}2 T cells

Effects of the static magnetic field generated by 0.5 T MRI unit on human CD4+ T cells activation patterns.

EFFECTS OF THE STATIC MAGNETIC FIELD GENERATED BY 0.5 T MRI UNIT ON INTRACELLULAR CALCIUM CONCENTRATION AND TNF-ALPHA RELEASE OF HUMAN MACROPHAGES

Analysis of memory and effector CD8 T cell subsets in chronic-Graft-versus-Host disease.

Effect of ultrasound contrast medium on human CD4+ T lymphocytes in vitro culture

Aminobisphosphonates as new weapons for gammadelta T Cell-based immunotherapy of cancer.

BACKGROUND: Activated V gamma 9 V delta 2 T cells are able to kill most tumour cells because of recognition by T cell receptor and natural killer receptors. OBJECTIVE: We discuss the possibility that the intentional activation of gammadelta T cells in vivo by aminobisphosphonates may represent a promising target for the design of novel and highly innovative immunotherapy in cancer patients. METHODS: The antitumoral effects of gammadelta T cells both in vitro and in vivo have been demonstrated suggesting a new therapeutic approach for translation into the clinical setting. RESULTS/CONCLUSION: V gamma 9 V delta 2 T lymphocytes represent a particularly interesting target for immunotherapeutic …

EFFECTS OF THE STATIC MAGNETIC FIELD GENERATED BY 0.5 T MRI UNIT ON INTRACELLULAR CALCIUM CONCENTRATION AND IFN-GAMMA RELEASE OF HUMAN CD4+ T CELLS.

Frequency, phenotype and function of Mycobacterium tuberculosis-specific CD8 T cells in patients with active tuberculosis and in individuals with latent infection

Effects of the static magnetic field generated by 0.5 T MRI unit on intracellular calcium concentration and TNF-α release of human macrophages

Homing and memory patterns of human gamma delta T cells in physiopathological situations

Memory and effector CD8 T cell subsets in human tuberculosis.

Effetti biologici del campo magnetico statico da 0,5 T generato da un tomografo RM sul rilascio di citochine da linfociti e macrofagi

EFFECTS OF THE STATIC MAGNETIC FIELD GENERATED BY 0.5 T MRI UNIT ON TNF-alpha RELEASE OF HUMAN PBMC FROM PATIENTS WITH RHEUMATOID ARTHRITIS.

Cross-talk between human myeloid dendritic cells and VG9VD2 T cells during M. tuberculosis infection.

Basi patogenetiche delle malattie autoimmuni

Comparative analysis of T cell recognition of conjugates containing genetically permissive epitope domain of the 38 kDa protein of M. tuberculosis

A novel ELISA system for detecting serum granulysin in tuberculosis patients

EFFECTS OF ELECTROMAGNETIC FIELDS ON IFN-GAMMA RELEASE AND CONTENENT FROM HUMAN SUBSETS OF CD4+T CELLS.

Differentiation of human Vgamma9 Vdelta 2 T cell subsets by antigen or homeostatic cytokines

The evolution of didactic programs of Italian medical faculties towards an European standard

Efficient cytotoxic activity of zoledronate-activated gamma delta T cells against imatinib CML cell lines.

Differentiation of human CD8+ T cells in BCG vaccinated and tuberculous patients.

Combining conventional chemotherapy and γδ T cell-based immunotherapy to target cancer-initiating cells.

Colon cancer comprises a small population of cancer initiating stem cells (CIC) that is responsible for tumor maintenance and resistance to anti-cancer therapies, possibly allowing for tumor recapitulation once treatment stops. Combinations of immune-based therapies with chemotherapy and other anti-tumor agents may be of significant clinical benefit in the treatment of colon cancer. However, cellular immune-based therapies have not been experimented yet in the population of colon CICs. Here, we demonstrate that treatment with low concentrations of commonly used chemotherapeutic agents, 5-fluorouracyl and doxorubicin, sensitize colon CICs to Vγ9Vδ2 T cell cytotoxicity. Vγ9Vδ2 T cell cytotoxi…

The expanding universe of gamma delta T lymphocytes: subsets, generation and function

Characterization of HLA-DR- and TCR-binding residues of an immunodominant and genetically permissive peptide of the 16-kDa protein of Mycobacterium tuberculosis

EFFECTS OF THE STATIC MAGNETIC FIELD GENERATED BY A 1.5 T MRI UNIT ON TNF ALFA RELEASE AND TNF RECEPTOR II EXPRESSION OF HUMAN MONOCYTES.

The unexpected functional plasticity of human γδ T cells: implications for immunotherapy and beyond.

Analysis of memory and effector CD8+ T cell subsets in chronic graft-versus-host disease

Effects of the static magnetic field generated by a 1,5 T MRI unit on TNF-a release and TNF-receptor II expression of human monocyes

Memory and effector CD8 T cell subset in human tuberculosis.

Novel insight into T cell immune response against Mycobacterium tuberculosis

Peptide conjugates as immune recognition based diagnostics.

Optimizing tumor-reactive γδ T cells for antibody-based cancer immunotherapy.

Monoclonal antibodies (mAbs) constitute the most rapidly growing class of human therapeutics and the second largest class of drugs after vaccines. The treatment of B-cell malignancies and HER2/Neu(+) breast cancer has benefited considerably from the use of therapeutic mAbs, either alone or in combination with standard chemotherapy. Frequent relapses, however, demonstrate that the bioactivity of these mAbs is still suboptimal. The concept of improving the anti-tumor activity of mAbs is well established and potentiating the cytotoxicity induced by anticancer mAbs can be achieved by strategies that target the downstream cytolytic effector cells. The recruitment of Fcγ receptor-dependent functi…

MONOCYTES MACROPHAGES EXPRESSION OF Ml OR M2 PHENOTYPES IN LATENT TUBERCULOSIS, ACTIVE DISEASES AND UNINFECTED MIGRANTS AND SICILIAN PATIENTS

The high grade ofphenotype plasticity of monocytes macrophages, is resumed in two different cell subsets named M1 or M2. Several studies of microbial infections in vitro and in vivo, showed that, during the early stage of infection, macrophages are polarized toward Ml phenotype that should be protective against pathogen, while during the chronic phase of infection/disease macrophages polarize toward M2 phenotype to avoid damages from a prolonged Ml type activation.Obiettivo: In order to investigate if Mycobacterium tuberculosis infection can drive circulating monocytes toward the expression of Ml or M2 phenotypes, we have analyzed by flow cytometry monocytes obtained from patients with acti…

Activation of human V gamma9 V delta 2 T cells by plasmacytoid dendritic cells

gammadelta T Cell Modulation in Anticancer Treatment

The broad antimicrobial and antitumoral reactivity of Vgamma9Vdelta2 T cells, their ability to produce inflammatory cytokines involved in protective immunity against intracellular pathogens and tumors and their strong cytolytic and bactericidal activities suggest their direct involvement in immune control of cancers and infections. gammadelta T cells can be selectively activated by naturally occurring or synthetic phosphoantigens, and drugs that enhance their accumulation into stressed cells, offering new avenues for the development of gammadelta T cell-based immunotherapies. The recent development of small drugs selectively activating Vgamma9Vdelta2 T lymphocytes, which upregulate endogeno…

CXCR5 identifies a subset of Vgamma9 Vdelta2 T cells which secrete IL-4 and IL-10 and help B cells for antibody production.

Vgamma9Vdelta2 T lymphocytes recognize nonpeptidic Ags and mount effector functions in cellular immune responses against microorganisms and tumors, but little is known about their role in Ab-mediated immune responses. We show here that expression of CXCR5 identifies a unique subset of Vgamma9Vdelta2 T cells which express the costimulatory molecules ICOS and CD40L, secrete IL-2, IL-4, and IL-10 and help B cells for Ab production. These properties portray CXCR5+ Vgamma9Vdelta2 T cells as a distinct memory T cell subset with B cell helper function.

Differential requirements for antigen or homeostatic cytokines for proliferation and differentiation of human Vgamma9Vdelta2 naive, memory and effector T cell subsets.

We have compared four human subsets of Vgamma9Vdelta2 T cells, naive (T(naive), CD45RA(+)CD27(+)), central memory (T(CM), CD45RA(-)CD27(+)), effector memory (T(EM), CD45RA(-)CD27(-)) and terminally differentiated (T(EMRA), CD45RA(+)CD27(-)), for their capacity to proliferate and differentiate in response to antigen or homeostatic cytokines. Cytokine responsiveness and IL-15R expression were low in T(naive) cells and progressively increased from T(CM) to T(EM) and T(EMRA) cells. In contrast, the capacity to expand in response to antigen or cytokine stimulation showed a reciprocal pattern and was associated with resistance to cell death and Bcl-2 expression. Whereas antigen-stimulated cells a…

HUMAN MEMORY VGAMMA9 VDELTA2 T CELLS REQUIRE HOMEOSTATIC CYTOKINES FOR PROLIFERATION AND DIFFERENTIATION.