0000000000435404

AUTHOR

Margus Rätsep

showing 3 related works from this author

Excitonic energy level structure and pigment-protein interactions in the recombinant water-soluble chlorophyll protein. II. Spectral hole-burning exp…

2011

Persistent spectral hole burning at 4.5 K has been used to investigate the excitonic energy level structure and the excited state dynamics of the recombinant class-IIa water-soluble chlorophyll-binding protein (WSCP) from cauliflower. The hole-burned spectra are composed of four main features: (i) a narrow zero-phonon hole (ZPH) at the burn wavelength, (ii) a number of vibrational ZPHs, (iii) a broad low-energy hole at ~665 and ~683 nm for chlorophyll b- and chlorophyll a-WSCP, respectively, and (iv) a second satellite hole at ~658 and ~673 nm for chlorophyll b- and chlorophyll a-WSCP, respectively. The doublet of broad satellite holes is assigned to an excitonically coupled chlorophyll dim…

ChlorophyllChlorophyll aExcitonAnalytical chemistryLight-Harvesting Protein ComplexesElectronsBrassicaVibrationSpectral linechemistry.chemical_compoundMaterials ChemistryPhysical and Theoretical ChemistryPhysics::Biological PhysicsChlorophyll AWaterFluorescenceRecombinant ProteinsSurfaces Coatings and FilmsWavelengthSpectrometry FluorescencechemistryExcited stateChlorophyllSpectral hole burningThermodynamicsAtomic physicsThe journal of physical chemistry. B
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Excitonic Energy Level Structure and Pigment−Protein Interactions in the Recombinant Water-Soluble Chlorophyll Protein. I. Difference Fluorescence Li…

2011

Difference fluorescence line-narrowing spectroscopy at 4.5 K was employed to investigate electron-phonon and electron-vibrational coupling strengths of the lower exciton level of water-soluble chlorophyll-binding protein (WSCP) from cauliflower reconstituted with chlorophyll a or chlorophyll b, respectively. The electron-phonon coupling is found to be moderate with integral Huang-Rhys factors S in the order of 0.81-0.85. A weak dependence of S on excitation wavelength within the inhomogeneously broadened fluorescence origin band is attributed to a sizable contribution of nonresonant excitation that varies with excitation wavelength. The strongly asymmetric and highly structured one-phonon p…

ChlorophyllChlorophyll bChlorophyll aChemistryPhononChlorophyll AExcitonLight-Harvesting Protein ComplexesAnalytical chemistryWaterElectronsBrassicaFluorescenceRecombinant ProteinsSurfaces Coatings and Filmschemistry.chemical_compoundSpectrometry FluorescenceChlorophyllMaterials ChemistryThermodynamicsPhysical and Theoretical ChemistrySpectroscopyExcitationThe Journal of Physical Chemistry B
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Red Spectral Forms of Chlorophylls in Green Plant PSI - A Site-Selective and High-Pressure Spectroscopy Study

2003

One of the special spectroscopic characteristics of photosystem I (PSI) complexes is that they possess absorption and emission bands at lower energy than those of the reaction center. In this paper, the red pigment pools of PSI-200, PSI-core, and LHCI complex from Arabidopsis thaliana have been characterized at low temperatures by means of spectrally selective (hole-burning and fluorescence line-narrowing) and high-pressure spectroscopic techniques. It was shown that the green plant PSI-200 complex has at least three red pigment pools, from which two are located in the PSI-core and one, in the peripheral light-harvesting complex I (LHCI). All of the red pigment pools are characterized by st…

Photosynthetic reaction centrePhysics::Biological PhysicsChlorophyll a/dk/atira/pure/sustainabledevelopmentgoals/affordable_and_clean_energyAnalytical chemistryAstrophysics::Cosmology and Extragalactic AstrophysicsPhotosystem IPhotosynthesisSurfaces Coatings and Filmschemistry.chemical_compoundPigmentchemistryAbsorption bandvisual_artMaterials Chemistryvisual_art.visual_art_mediumAstrophysics::Solar and Stellar AstrophysicsSDG 7 - Affordable and Clean EnergyPhysical and Theoretical ChemistrySpectroscopyAbsorption (electromagnetic radiation)
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