Monitoring the Shelf-Life of Minimally Processed Fresh-Cut Apple Slices By Physical–Chemical Analysis and Electronic Nose

Fresh-cut apples, in slices or in cubes, are minimally processed products, which are currently collecting a great interest by fruit marketers for their promising diffusion. Their shelf life, from a microbiological point of view, has been fixed about 2 or 3 weeks under refrigeration. However in a few days they undergo biochemical degradations with production of off-flavors and texture breakdown. In this work, the change of aromatic fingerprint of apple slices packaged in air and in a modified atmosphere (with 100% N2) and stored at 4°C was measured, by using a commercial electronic nose. The obtained data were also compared with sensory evaluation of judge’s panel. Moreover, quality paramete…

Crioconservazione di germoplasma di Citrus spp.

Monitoring and cellular characterisation of male gametic embryogenesis in Citrus clementina.

Correlation of sequentila floral development and male gametophyte development and preliminary results on anther culture in Opuntia ficus-indica. Acta Physiologiae Plantarum Vol. 27 No. 4B:

STUDY ON ISOLATED MICROSPORE CULTURE IN CITRUS SINENSIS L. OSBECK CV. MORO, A BLOOD ORANGE CULTIVAR

In vitro tissue culture represents a useful support for the advancement of Citrus breeding and propagation. Haploidy technology, that is the single-step development of complete homozygous genotypes from heterozygous parents through gametic embryogenesis, has already a huge impact on many relevant crops, representing an integral part in their breeding programmes (Germanà 2011a; 2011b). In order to increase the number of genotypes responding to gametic embryogenesis, in vitro isolated microspore culture of Citrus sinensis L. Osbeck cv. Moro, has been carried out, investigating the influence of the culture medium composition, and, particularly, of the plant growth regulator types and concentra…

Proteomic approach to characterize homozygous Citrus clementina Hort. ex Tan. Genotypes

Anther culture for haploid and doubled haploid production From the issue entitled "Special Issue: In Vitro Ploidy Manipulation in the Genomics Era"

CHARACTERISATION OF MICROSPORE EMBRYOGENESIS IN ANTHER CULTURE OF CITRUS.

Analisi dei trascritti caratterizzanti il processo di maturazione in ficodindia (Opuntia ficus-indica L.)

FIRST STAGES OF MICROSPORE REPROGRAMMING TO EMBRYOGENESIS THROUGH ISOLATED MICROSPORE CULTURE IN Eriobotrya japonica LINDL.

Miglioramento delle tecniche di propagazione e conservazione in vitro dell'olivo.

Il miglioramento genetico degli agrumi: metodi tradizionali e innovativi.

Rigenerazione da coltura in vitro di antere di agrumi.

“La conservazione ex situ dei fruttiferi mediante coltura in vitro: applicazione della crescita rallentata e della crioconservazione al nocciolo e al ciliegio”

Evaluation of the ligno-cellulosic residuals of a suburban citrus orchad for energy generation.

Coltura in vitro di microspore isolate per il miglioramento genetico di Olea europaea L.

Gli aploidi (piante con un numero gametofitico di cromosomi nello sporofito: n invece di 2n) ed i doppio-aploidi (aploidi che hanno raddoppiato il numero di cromosomi spontaneamente o in maniera indotta) trovano numerose applicazioni nel miglioramento genetico vegetale, ed in particolare, nella mutagenesi, nella selezione in vitro, nella trasformazione, nell’utilizzo della variabilità gametoclonale ed anche nel sequenziamento del genoma e negli studi di genomica funzionale. Pertanto, essi costituiscono uno strumento biotecnologico particolarmente interessante nel miglioramento genetico delle piante. Ai fini dell’ottenimento dell’omozigosi nell’olivo, come in tutte le piante arboree, caratte…

Studio preliminare sulla germinazione in vitro di semi di Argania spinosa (L.)

CHARACTERIZATION OF LEAF ESSENTIAL OIL COMPOSITION OF HOMOZYGOUS AND HETEROZYGOUS Citrus clementina HORT. EX TAN. AND OF ITS ANCESTORS

Research on essential olis of 32 Citrus limon L. Burm. clones

Effects of high and low intensità magnetic fields on pollen behaviour of Citrus clementina Hort. ex Tan. and Pinus pinea L.

Radicazione ed incapsulamento di talee vitro-derivate di citrange [Citrus sinensis (L.) Osb. X Poncirus trifoliata (L.) Raf.] Carrizo.

ANTHER CULTURE TECHNIQUE FOR BREEDING AND PROPAGATION IN CITRUS AND IN OTHER MEDITERRANEAN FRUIT TREES

Ex vitro mycorrhization of vitro-derived plantlets of 'Carrizo' citrange [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]

In this study, vitro-derived plantlets of 'Carrizo' citrange were inoculated with Glomus intraradices during the acclimatization phase, from in vitro to in vivo. Plantlets were grown in presence (+M) and in absence of inoculum (-M) and in addition, in sterile and non-sterile substrate. After three months of culture, some vegetative parameters and the level of infection, varied greatly in the different theses. In particular, the highest formation of new branched roots has been observed in inoculated plantlets cultivated in sterile substrate. Good results were observed also in non-inoculated plantlets cultivated in non-sterile substrate, in presence of endemic inoculum. The same trend was obs…

RICERCHE SULLA VARIAZIONE DELLA FRAZIONE VOLATILE IN FOGLIE DI 14 CULTIVAR DI OLIVO IN RELAZIONE ALL’EPOCA DI PRELIEVO

Primeras etapas de la embriogenesis de microsporas en coltivo in vitro de anteras de Citrus clementina Hort. ex Tan.

Anther and isolated microspore culture in the fruit tree species Prunus avium L .

Studio sull’incapsulamento di microtalee vitro-derivate di gelso.

Studio preliminare sulle caratteristiche qualitative e salutistiche di 4 cultivar di mango (Mangifera indica L.) coltivate in Sicilia.

Study on isolated microspore culture in Citrus sinensis Osbeck cv. Moro, a blood orange cultivar].

In vitro establishment and culture of two Sicilian cultivars of Olea europaea L

Sicily is among the Italian regions with the largest cultivation area of olive (Olea europaea L.). The applications of in vitro tissue culture, as practical tool of propagation and germplasm storage, were not sufficiently exploited in this species, because of the lack of efficient in vitro establishment protocols and culture for the majority of the cultivars. In fact, it is well known that the success of in vitro culture is strongly dependent on the species and, within the species, on the cultivars. This study reports the in vitro establishment of two Sicilian olive cultivars, grown in greenhouse. Both genotypes showed a percentage of in vitro sprouting higher than that one previously obtai…

Morphological and Anatomical Observations of Abnormal Somatic Embryos from Anther Cultures of Citrus reticulata Blanco.

The oil composition of homozygous and heterozygous plants of Citrus clementina Hort. ex Tan.".

Morphogenic response in vitro of epicotyl segments of Citrus macrophylla.

Preliminary results on anther culture in Opuntia ficus-indica.

Oil composition of homozygous and heterozygous plants of Citrus clementina Hort. ex Tan.

Breeding of Orchids Using Conventional and Biotechnological Methods: Advances and Future Prospects

The domestication of plants, including ornamental plants, is an ancient tradition based on human curiosity and is the first form of plant breeding based on modern agriculture. However, recent advances in biotechnology, molecular biology, and genetic engineering, associated with better knowledge of the genome, have enabled numerous achievements to advance agriculture towards a new green revolution. In orchids, it has not been different, and recent advances in genome sequencing and the development of more sophisticated breeding techniques based on biotechnology have demonstrated their applicability for the constitution of more sophisticated cultivars. However, conventional breeding by directe…

ISOLATION AND CHARACTERIZATION OF RNA FRACTIONS INVOLVED IN THE MATURATION PROCESS IN OPUNTIA FICUS-INDICA (L.) MILL.

Diversità e sviluppo dell'agrumicoltura mondiale all'XI Citrus Congress

FIRST EMBRYONIC STAGES OF CITRUS MICROSPORE DERIVED EMBRYOS.

Naso Elettonico con sensori MOS per discriminare 3 cultivar di Eriobotrya japonica Lindl.

Extraction of Proteins From Leaves of Homozygous and Heterozygous Citrus for Two-Dimensional Electrophoresis Analysis.

Studio sulla germinabilità in vitro di semi di Fraxinus angustifolia Vahl.

Osservazioni sulla radicazione di talee di genotipi di Olea europaea var. sylvestris

Studio sull'embriogenesi gametica in Olea europaea L.

ISOLATED MICROSPORE CULTURE IN THE FRUIT TREE SPECIES PRUNUS AVIUM, ERIOBOTRYA JAPONICA, OLEA EUROPAEA AND OPUNTIA FICUS-INDICA.

Morfogenesi in vitro e seme sintetico come strumento innovativo per lapropagazione e conservazione del germoplasma delle piante arboree mediterranee

IN VITRO GERMINATION AND SEEDLING DEVELOPMENT OF CAPER (CAPPARIS SPINOSA L.)

Observations on the effects of high and low intensity magnetic fields on pollen behaviour of Citrus Clementina Hort.ex Tan.and Pinus pinea L.

Haploid technology in fruit tree breeding

In Vitro Plant Regeneration from Epicotyls Segments of Carrizo Citrange.

Naso elettronico con sensori Mos per discriminare 3 cultivar di Eriobotrya japonica Lindl.

Prospettive della tecnologia dell’incapsulamento nel vivaismo agrumicolo.

Haploidy. In: Khan I. (Ed.) Citrus. Genetics, breeding and biotechnology.

Attività antifungina e composizione chimica di oli essenziali estratti da agrumi coltivati in Sicilia.

Risulta comprovata nell’industria agroalimentare l’efficacia di oli essenziali di agrumi quale tecnologia per esaltare la sapidità di cibi e bevande. Ad oggi, il rinnovato interesse verso gli oli essenziali deriva dalle loro proprietà antibatteriche ed antifungine, che possono trovare ampia applicazione in sistemi hurdle-technology ai fini di prolungare la shelf-life di agroprodotti, specie in cibi minimally-processed e di IV gamma. Infatti, la necessità di ricercare nuove sostanze non di sintesi con proprietà antimicrobiche deriva dai cambiamenti legislativi in campo alimentare, dalla tendenza dei consumatori ad acquistare cibi processati naturalmente e dalla crescente resistenza dei patog…

EFFECTS ON SEED GERMINATION OF DIFFERENT BIOCHARS FROM INDUSTRIAL THERMOCHEMICAL PROCESSES

Char or biochar is a type of charcoal obtained from gasification/pyrolysis of biomasses. Instead of burning standing biomass from cleared forest, the resource is charred. The result is a highly porous, carbon-rich solid residue, really similar in appearance to the coal produced by natural burning. First considered an industrial waste, in recent years, the interest in this material has grown enormously given its ability to improve physical, chemical, biological and mechanical properties of soils, when used as amendment. However, its effects are highly variable depending onits chemical-physical properties which in turn depend greatly on the starting feedstock. The present study reports about …

IN VITRO GERMINATION OF STONELESS AND ISOLATED EMBRYOS OF 14 SICILIAN CULTIVARS OF OLEA EUROPAEA L.

Seasonal variations of antimicrobial activity and chemical composition of Citrus limon L. Burm. spp. essential oils

The interest towards the application of essential oils (EOs) is increasing due to the antimicrobial potential to be used as “natural” alternative to the chemical preservation of foods [1]. EOs are aromatic oily liquids produced as secondary metabolites in various plants exerting different biological properties, such as antibacterial, antiviral, antimycotic and insecticidal. EOs are complex mixtures of lipophilic substances and the chemical composition, thereby the biological activities, is influenced by the raw plant material (genotype, part of the plant, harvest time, geographical, ecological conditions and cultural techniques) and extraction method [2].

Qualità dei frutti di Mango prodotti in Sicilia

Confronto tra la frazione volatile in foglie di cultivar di Olea europea L. in Sicilia

Gametic and somatic embryogenesis through anther culture in Citrus.

Preliminary results on the effect of light quality on anther culture of Citrus clementina.

GAMETIC EMBRYOGENESIS IN CITRUS.

Microspore-derived embryo recovery trough isolated microspore culture in Corylus avellana L.

Valutazione della variabilità genetica di alcune cultivar di nespolo del Giappone mediante SSRs e AFLP..

Studio preliminare sulla coltura in vitro di due genotipi siciliani di Morus.

Observations on anatomical structures of in vitro germination of stoneless and isolated olive embryos.

Development of multi-cellular pollen of Eriobotrya japonica through anther culture.

Caratterizzazione molecolare di un mutante di Olea europaea L. a taglia ridotta.

Studio sul potenziale morfogenico di segmenti di epicotile di Citrus macrophylla Wester.

La tecnologia dell'incapsulamento nella gestione del germoplasma vegetale

Prove sulla radicazione di talee di cultivar siciliane di Olea europaea L.

Organizzazione della filiera vivaistica e certificazione delle piante.

Le tecniche di moltiplicazione, l'uso del portinnesto, la micropropagazione: molti gruppi di ricerca in Italia sono attivi nel miglioramento dei sistemi di propagazione del nocciolo. Avanzano anche i lavori per lo sviluppo del programma di certificazione genetico sanitaria, su base volontaria, del materiale vivaistico che, alla luce del mutato quadro normativo europeo, dovrebbe essere operativo entro la fine del 2017.

GAMETIC AND SOMATIC EMBRYOGENESIS BY ANTHER CULTURE TECHNIQUE IN CITRUS AND IN OTHER MEDITERRANEAN FRUIT TREES

The effects of benzyladenine and meta-Topolin on in vitro sprouting and regrowth after encapsulation of C35 citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf] microcuttings

C35, a hybrid of ‘Ruby Blood’ sweet orange and trifoliate orange, is a promising Citrus rootstock, mainly due to its resistance to Citrus Tristeza Virus (CTV), one of the most serious pathology affecting citriculture. The use of resistant rootstocks, such as citranges, is an efficient tool to fight this plant disease. This implies a continuous increasing demand of resistant rootstocks, in order to carry out the turnover of sour orange, still the most used Citrus rootstock in Italy. Micropropagation, can provide innovative solutions to obtain, in a short time and in a small space, a number of plants higher than traditional propagation. Micropropagation efficiency is also affected by the cult…

Advancement on isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. Monreal Rosso and Nules.

Anther culture in Citrus:a powerful tool to recover seedless triploids

Coltura in vitro di antere per l'ottenimento di embriogenesi gametica e somatica in Citrus

Discrimination of three Citrus paradisi Macf. cultivars by Electronic Nose Discrimination of three Citrus paradisi Macf. cultivars by Electronic Nose.

Ottenimento di triploidi mediante coltura in vitro di semi di Citrus clementina Hort. ex Tan., cv. Monreal Bianco e Monreal Verde.

Seme sintetico negli agrumi: stato attuale delle ricerche

Study on anther culture in hazelnut (Corylus avellana L.) .

Research on the volatile fraction in leaves of Olea europaea subsp. oleaster and Olea europaea subsp. sativa.

Doubled Haploid Technology in fruit crops.

Preliminary studies on encapsulation of gametic and somatic embryos of Citrus clementina and Citrus reticulata: effect of cold storage

Embriogenesi da coltura in vitro di antere di Citrus

Stabilizzazione e coltura in vitro di due genotipi siciliani di olivo (Olea europaea L.)

Utilizzo dell’embriogenesi gametica per il miglioramento genetico dei Citrus.

ANTHER CULTURE TECHNIQUE TO OBTAIN GAMETIC AND SOMATIC EMBRYOGENESIS IN CITRUS.

Studio sulla coltura in vitro in Punica granatum L.

Molecular and cytological characterization of homozygous plants of Citrus clementina Hort. ex Tan., candidates to citrus genome sequencing. .

Correlation of sequential floral and male gametophyte development in Opuntia ficus-indica.

The wild olive of Sicily, Their past and potential contribution to the improvement of the cultivated varieties

Preliminary Results on Carrizo Citrange Synthetic Seeds Dessiccation and PVS2-Vitrification as Pre-Treatments To Cryopreservation

Studio di protocolli efficienti da utilizzare per la propagazione in vitro di cultivar siciliane di olivo (Olea europaea L.) mediante lo studio dei fattori che influenzano il successo della coltura.

Esperienze di incapsulamento di propaguli vitro-derivati di germoplasma vegetale

Analisi della variazione del contenuto in polifenoli totali del succo dei frutti di tre varietà di limone (Citrus limon L. Burm) coltivate in Sicilia

Embryo production through isolated microspore culture in Citrus clementina Hort. ex Tan., cv. Monreal Rosso .

Haploid and Doubled Haploids in Fruit Trees.

Discrimination of hand fresh squeezed and commercial grapefruit juices by electronic nose.

Use of irradiated pollen to induce Parthenogenesis and haploid production in fruit crops.

First stages of microspore reprogramming to embryogenesis through anther culture in Prunus armeniaca L

Correlation of sequential floral development and male gametophyte development and preliminary results on anther culture in Opuntia ficus-indica.

Organogenesi in vitro da porzioni di epicotile di citrange Troyer (Citrus sinensis [L.] Osbeck x Poncirus trifoliata L. Raf.)

In vitro establishment and culture of several Sicilian olive genotypes (Olea europaea L.)

Biotechnology applications to conservation and improvement of Mediterranean fruit crops.

Analisis genetico de regenerantes de Citrus reticulata Blanco obtenidos por coltivo in vitro de anteras.

In vitro anther culture of several cultivars of Citrus sinensis and Citrus clementina.

COLTURA IN VITRO DI ANTERE E MICROSPORE ISOLATE PER IL MIGLIORAMENTO GENETICO DEGLI AGRUMI E DI ALTRE PIANTE ARBOREE MEDITERRANEE

Study on the effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan.

Preliminary Results on Biotization of Encapsulated Vitro-Derived Propagules of Carrizo Citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf].

The encapsulation technology represents a new tool to integrate micropropagation into the nursery activity. It allows combining the advantages of zygotic or gamic seeds with those of micropropagation. The synthetic or artificial seeds have been defined as “artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions”. This will be a powerful propagation tool in the nurseryman hands, if the levels of the synthetic seeds conversion can be increased also in the nurseries, without the asepsis of in vitro laboratories and with the presence of many parasitic microorganisms, like bacteria and fungi, responsible for contaminat…

Use of gametic embryogenesis for Citrus improvement.

Study on flavonoid contents in fruits of different Citrus genotypes

Contenuto lipidico ed acidi grassi del polline di diversi genotipi di agrumi