0000000000443122
AUTHOR
Anna Ribbene
Evalutaion of HSP60, Procaspase-3 and P53 expression after oxydative stress in NCI-H292 cells.
Chaperonology: A novel research field for experimental medicine in the XXI century.
ADAM33, alpha smooth muscle actin and collagen expression in human embryonic lung in vivo and in culture.
Extracellular release of HSP60 from tumor cells occurs via various secretory pathways
Hsp60 is actively secreted by human tumor cells
Background Hsp60, a Group I mitochondrial chaperonin, is classically considered an intracellular chaperone with residence in the mitochondria; nonetheless, in the last few years it has been found extracellularly as well as in the cell membrane. Important questions remain pertaining to extracellular Hsp60 such as how generalized is its occurrence outside cells, what are its extracellular functions and the translocation mechanisms that transport the chaperone outside of the cell. These questions are particularly relevant for cancer biology since it is believed that extracellular chaperones, like Hsp70, may play an active role in tumor growth and dissemination. Methodology/Principal Findings S…
Evaluation of hsp60, procaspase-3 and p53 expression after oxydative stress in H292 cells
Oxidative stress apoptosis in carcinogenesis: molecular players and interactions.
HSP60 and HASP10 overexpression in metastatic colon cancer
EXTRACELLULAR RELEASE OF HSP60 FROM TUMOR CELLS
Evaluation od hsp60, pro-caspase-3 and p53 expression after oxidative stress in NCI-H292 cells
Upon oxidative stress, the antiapoptotic Hsp60/procaspase-3 complex persists in mucoepidermoid carcinoma cells.
Hsp60, a mitochondrial chaperonin highly conserved during evolution, has been found elevated in the cytosol of cancer cells, both in vivo and in vitro, but its role in determining apoptosis during oxidative stress (OS) has not yet been fully elucidated. The aim of the present work was to study the effects of OS on Hsp60 levels and its interactions with procaspase- 3 (p-C3) and p53 in tumor cells. NCI-H292 (mucoepidermoid carcinoma) cells were exposed to various concentrations of hydrogen peroxide (H2O2) for 24 hours. Cell viability was determined by Trypan blue and MTT assays. DNA damage was assessed by the Comet assay, and apoptosis was measured by the AnnexinV cytofluorimetric test. Expos…
STUDYING EMBRYONIC LUNG DEVELOPMENT BY TISSUE ENGINEERING.
Multi-chaperone – interactors network (mtcin) in mitochondria: role in neoplasm development and methodology of analysis.
Hsp60 and Hsp10 overexpression in metastatic colon cancer.
ANP and CD34 expression in human dilated cardiomiopathies: a new insight
Chronological expression of Ciliated Bronchial Epithelium 1 during pulmonary development
Ciliated Bronchial Epithelium (CBE) 1 is a novel gene, which is expressed in ciliated cells. As cilia are important during embryogenesis, the present authors characterised the murine homologue of CBE1 (Cbe1) and compared its temporal expression during murine and human lung development. Cbe1 cDNA was cloned and characterised using sequencing, standard PCR and Western blotting. Mouse and human embryonic/fetal lungs (HELs) were harvested for mRNA analysis and protein localisation in vivo and in vitro using RT-PCR and immunohistochemistry. The Cbe1 amino acid sequence was >75% identical with CBE1 and its alternative splicing and tissue distribution were highly conserved. Pulmonary expression of…