Gel chromatography

Abstract The separation efficiency of gel chromatographic columns was investigated under a wide variety of experimental conditions. By use of reduced quantities it is possible to describe the separation efficiency for different gels and experimental conditions by a single equation. Deviations from this equation were found to be caused by an alteration of the diffusion coefficient within the gel with respect to the mobile phase. Factors governing the gel chromatography of p -oligophenylenes on poly(vinyl acetate) and poly(styrene) gels are generalized and discussed.