0000000000454400
AUTHOR
Detlef Stöckigt
High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionisation mass spectrometric analysis of selected alkaloid groups
Systems for efficient separation of selected alkaloid groups by high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and capillary electrophoresis coupled with electrospray ionisation mass spectrometry (CE-ESI-MS) are described. The optimized HPLC system was applied for the separation of 23 standard indole alkaloids as well as for qualitative and quantitative analyses of crude alkaloid extracts of Rauvolfia serpentina X Rhazya stricta hybrid cell cultures. The developed conditions for CE analysis proved to be efficient for separation of mixtures of standard indole and beta-carboline alkaloids. The described buffer system is also applicable in the combination of CE w…
General approach for the analysis of various alkaloid classes using capillary electrophoresis and capillary electrophoresis-mass spectrometry
Abstract The analysis of various alkaloid classes employing capillary electrophoresis (CE) and on-line combined CE-mass spectrometry (CE-MS) is described. A CE method is presented for the analysis of alkaloids without derivatisation or purification. The separation of four different groups of alkaloids consisting of monoterpenoid indole alkaloids, protoberberines/benzophenanthridines, β-carboline alkaloids, and isoquinolines from poppy by free zone capillary electrophoresis has been obtained using a 1:1 mixture of 100 mmol 1 −1 ammonium acetate (pH 3.1) and acetonitrile. The influence of alkaloid structure on the electrophoretic mobility is discussed. The CE-MS reconstructed total ion curren…
Analysis of Rauwolfia Alkaloids Employing Capillary Electrophoresis-Mass Spectrometry
Abstract Capillary electrophoresis-mass spectrometry (CE-MS) was applied to analyse extracts of roots and cell suspension cultures from Rauwolfia serpentina. Most of the alkaloids known to be present in the respective plant material have been resolved via CE and assigned according to their electrospray mass spectra.