0000000000454575

AUTHOR

Klara Brixius

showing 2 related works from this author

Regulation of endothelial nitric oxide synthase (eNOS) in myocardium subjected to cardioplegic arrest.

2009

BACKGROUND: Nitric oxide (NO) production by both coronary endothelial cells and cardiomyocytes is thought to play a significant role in myocardial pathophysiology following ischemia/reperfusion (I/R). METHODS: In thirteen pigs subjected to 1 hour cardioplegic arrest (CA) on CPB, left ventricular (LV) biopsies were collected prior to CPB (baseline), at 60 min CPA, at 15 and 30 min reperfusion on CPB, and at 120 min post CPB. LV specimens were immunocytochemically stained against phospho-eNOS Ser1177 , phospho-eNOS Thr495 , phosphorylated ERK1/2, and AKT/PKB. Four additional pigs without CA served as controls. Cardiomyocytes were quantitatively investigated using TV densitometry (gray units: …

Pulmonary and Respiratory MedicineMaleThreoninemedicine.medical_specialtyTime FactorsNitric Oxide Synthase Type IIISwineHeart VentriclesIschemiaEnos phosphorylationVentricular Function LeftNitric oxidechemistry.chemical_compoundEnosInternal medicinemedicineSerineAnimalsPhosphorylationProtein kinase BMitogen-Activated Protein Kinase 1Cardiopulmonary BypassMitogen-Activated Protein Kinase 3Endothelial nitric oxide synthasebiologybusiness.industryMyocardiummedicine.diseasebiology.organism_classificationImmunohistochemistryMyocardial ContractionPathophysiologyEnzyme ActivationEndocrinologychemistryModels AnimalCardiologyHeart Arrest InducedPhosphorylationSurgeryFemaleCardiology and Cardiovascular MedicinebusinessProto-Oncogene Proteins c-aktThe Thoracic and cardiovascular surgeon
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Extracorporeal circulation activates endothelial nitric oxide synthase in erythrocytes.

2007

Background Extracorporeal circulation used in cardiopulmonary bypass and hemodialysis is often associated with severe hypotension, which is an important predictor for mortality and morbidity. One pathophysiological hypothesis includes nitric oxide (NO) generation. Recently, a functional NO synthase (endothelial type NO synthase [eNOS]), was found to be expressed in human red blood cells. However, to date, activation of red blood cell eNOS has not been shown. We hypothesized that eNOS in circulating red blood cells might be activated during extracorporeal circulation and thus contribute to hypotension through vasodilation upon NO release. Methods We collected blood samples from 28 patients e…

Pulmonary and Respiratory Medicinemedicine.medical_specialtyErythrocytesEndotheliumNitric Oxide Synthase Type IIIVasodilationNitric Oxidelaw.inventionNitric oxidechemistry.chemical_compoundlawEnosInternal medicinemedicineCardiopulmonary bypassHumansCardiopulmonary Bypassbiologybusiness.industryExtracorporeal circulationbiology.organism_classificationNitric oxide synthaseEnzyme ActivationVasodilationRed blood cellEndocrinologymedicine.anatomical_structurechemistryAnesthesiabiology.proteinSurgeryHypotensionCardiology and Cardiovascular MedicinebusinessThe Annals of thoracic surgery
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