Protocol to compare column performance applied to hydrophilic interaction liquid chromatography

Abstract The lack of retention of highly polar solutes in liquid chromatography with reversed phase columns (RPLC) can be solved through the use of the hydrophilic interaction liquid chromatographic (HILIC) mode. Due to the complexity of the separation mechanisms in HILIC and the different factors that may have significant influence, the selection of the appropriate stationary phase plays a fundamental role in the development of analytical procedures to obtain good performance. In this work, the chromatographic performance of bare silica and six polar stationary phases with different functionalized groups (with neutral, cationic, anionic and zwitterionic character) are investigated. The beh…