Toxoplasmosis after hematopoietic stem cell transplantation.
In immunocompromised individuals, toxoplasmosis mostly occurs as a reactivation of a latent infection, causing severe to life-threatening disease. Thus, recipients who are seropositive for Toxoplasma gondii before an allogeneic hematopoietic stem cell transplant (HCT) are at highest risk, although primary infections may also cause severe toxoplasmosis. The disease most often affects the central nervous system, but in HCT recipients other organs are involved in more than half of the cases. Because of the alteration of the immune response in these patients, serodiagnosis is not sufficiently reliable in the diagnosis of post-HCT toxoplasmosis, and direct detection of the causative agent is req…
Méthodes d’identification et de quantification des moisissures de l’habitat : méthodes classiques, méthodes moléculaires
Resume Introduction Pour apprehender les repercussions de l’inhalation constante et inevitable de spores de moisissures il est necessaire de les prelever, les identifier et les quantifier. Etat des lieux Les prelevements sont de trois types : (i) surfaces, de realisation facile mais non quantifiables, (ii) air, faciles a calibrer mais limites dans le temps, et (iii) poussieres, plus representatifs dans le temps d’une exposition aux moisissures. La strategie d’echantillonnage depend des objectifs (exposition des personnes, objectiver la contamination, efficacite de la remediation). L’identification des colonies obtenues en culture est realisee par microscopie, Maldi-TOF, et/ou par sequencage…
Toxoplasmosis after hematopoietic stem transplantation. Report of a 5-year survey from the Infectious Diseases Working Party of the European Group for Blood and Marrow Transplantation
Toxoplasmosis after hematopoietic stem transplantation. Report of a 5-year survey from the Infectious Diseases Working Party of the European Group for Blood and Marrow Transplantation
Early detection of Toxoplasma infection by molecular monitoring of Toxoplasma gondii in peripheral blood samples after allogeneic stem cell transplantation
International audience; Background. Isolated case reports have shown that recipients of allogeneic hematopoietic stem cell transplants ( HSCTs) who develop toxoplasmosis may have circulating Toxoplasma gondii DNA in peripheral blood before the onset of clinical symptoms. Methods. We prospectively studied 106 T. gondii - seropositive adult recipients of HSCTs for the incidence of reactivation of toxoplasmosis in the first 6 months after transplantation. Toxoplasmosis infection ( TI) was defined by a positive result of polymerase chain reaction ( PCR) of peripheral blood specimens, whereas toxoplasmosis disease ( TD) was defined as an invasive infection. Results. The incidence of TI was 16% (…