0000000000462954

AUTHOR

Sakari Mäntyselkä

showing 3 related works from this author

Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes

2022

Impaired lipid metabolism is a common risk factor underlying several metabolic diseases such as metabolic syndrome and type 2 diabetes. Branched-chain amino acids (BCAAs) that include valine, leucine and isoleucine have been proven to share a role in lipid metabolism and hence in maintaining metabolic health. We have previously introduced a hypothesis suggesting that BCAA degradation mechanistically connects to lipid oxidation and storage in skeletal muscle. To test our hypothesis, the present study examined the effects of BCAA deprivation and supplementation on lipid oxidation, lipogenesis and lipid droplet characteristics in murine C2C12 myotubes. In addition, the role of myotube contract…

metabolic healthEXERCISEaminohapotMETABOLISMDEHYDROGENASE COMPLEXSUPPLEMENTATIONrasva-aineenvaihduntain vitro exerciseALPHAACTIVATIONMICEaineenvaihduntahäiriötCONTRACTIONelectrical pulse stimulationSKELETAL-MUSCLE1182 Biochemistry cell and molecular biologylipids (amino acids peptides and proteins)skeletal muscleaikuistyypin diabetesOBESElihassolutprotein supplementationnuclear magnetic resonance spectroscopy
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Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation

2021

The application of exercise-like electrical pulse simulation (EL-EPS) has become a widely used exercise mimetic in vitro. EL-EPS produces similar physiological responses as in vivo exercise, while less is known about the detailed metabolic effects. Routinely, the C2C12 myotubes are cultured in high-glucose medium (4.5 g/L), which may alter EL-EPS responses. In this study, we evaluate the metabolic effects of EL-EPS under the high- and low-glucose (1.0 g/L) conditions to understand how substrate availability affects the myotube response to EL-EPS. The C2C12 myotube, media, and cell-free media metabolites were analyzed using untargeted nuclear magnetic resonance (NMR)-based metabolomics. Furt…

0301 basic medicinemedicine.medical_specialtyasetaatitbranched chain fatty acidsPhysiologyEndocrinology Diabetes and MetabolismMuscle Fibers SkeletalrasvahapotStimulationglukoosi03 medical and health sciencesMice0302 clinical medicineMetabolomicsPhysiology (medical)Internal medicinePhysical Conditioning AnimalMetabolomemedicineAnimalsskeletal muscleaineenvaihduntalihassolutCells CulturedsolufysiologiaChemistryPulse (signal processing)MyogenesisSkeletal muscleBranched chain fatty acidsmetabolomicslaktaatitElectric Stimulation030104 developmental biologymedicine.anatomical_structureEndocrinologyGlucosein vitro -menetelmäaineenvaihduntatuotteetacetateexerkineC2C12030217 neurology & neurosurgeryAmino Acids Branched-ChainResearch Article
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The metabolic responses to electrical pulse-induced contractions in C2C12 myotubes are greater in high glucose medium

2021

Osaa liikunnan terveyshyötyjen mekanismeista on vaikeaa tutkia ihmisillä tai koe-eläimillä. Viljeltyjen C2C12 myotuubien sähköpulssistimulaatiolla (EPS, engl. electrical pulse stimulation) voidaan mallintaa lihassupistuksia ja liikuntaa in vitro –olosuhteissa. EPS:n vaikutusta C2C12 myotuubien aineenvaihduntaan ei kuitenkaan tunneta tarkasti. Lisäksi C2C12 myotuubeja viljellään yleensä korkeassa ei-fysiologisessa 25 mM glukoosipitoisuudessa, mikä voi vaikuttaa EPS-vasteisiin. Tässä tutkimuksessa C2C12 myotuubeja sähköpulssistimuloitiin 24 tunnin ajan sekä matalan 5.5 mM (MG) että korkean 25 mM (KG) glukoosipitoisuuden olosuhteissa, ja aineenvaihduntatuotteiden konsentraatiot analysoitiin se…

amino acidsexercise modelBSCFAsrasvahapotACOT9lihaksetsolumallitaminohapotmetabolomicsfatty acidscell modelsglukoosimusclesskeletal muscleglucoseaineenvaihduntametabolism
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