Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes

6533b7d0fe1ef96bd125a0b9

RESEARCH PRODUCT

Branched-Chain Amino Acid Deprivation Decreases Lipid Oxidation and Lipogenesis in C2C12 Myotubes

Sira Karvinen Vasco Fachada Ulla-maria Sahinaho Satu Pekkala Juulia H. Lautaoja Sakari Mäntyselkä Perttu Permi Juha J. Hulmi Mika Silvennoinen Heikki Kainulainen

subject

metabolic health EXERCISE aminohapot METABOLISM DEHYDROGENASE COMPLEX SUPPLEMENTATION rasva-aineenvaihdunta in vitro exercise ALPHA ACTIVATION MICE aineenvaihduntahäiriöt CONTRACTION electrical pulse stimulation SKELETAL-MUSCLE 1182 Biochemistry cell and molecular biology lipids (amino acids peptides and proteins)skeletal muscle aikuistyypin diabetes OBESE lihassolut protein supplementation nuclear magnetic resonance spectroscopy

description

Impaired lipid metabolism is a common risk factor underlying several metabolic diseases such as metabolic syndrome and type 2 diabetes. Branched-chain amino acids (BCAAs) that include valine, leucine and isoleucine have been proven to share a role in lipid metabolism and hence in maintaining metabolic health. We have previously introduced a hypothesis suggesting that BCAA degradation mechanistically connects to lipid oxidation and storage in skeletal muscle. To test our hypothesis, the present study examined the effects of BCAA deprivation and supplementation on lipid oxidation, lipogenesis and lipid droplet characteristics in murine C2C12 myotubes. In addition, the role of myotube contractions on cell metabolism was studied by utilizing in vitro skeletal-muscle-specific exercise-like electrical pulse stimulation (EPS). Our results showed that the deprivation of BCAAs decreased both lipid oxidation and lipogenesis in C2C12 myotubes. BCAA deprivation further diminished the number of lipid droplets in the EPS-treated myotubes. EPS decreased lipid oxidation especially when combined with high BCAA supplementation. Similar to BCAA deprivation, high BCAA supplementation also decreased lipid oxidation. The present results highlight the role of an adequate level of BCAAs in healthy lipid metabolism. Peer reviewed

year	journal	country	edition	language
2022-04-01

10.3390/metabo12040328 http://hdl.handle.net/10138/343560

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