Identification of gene expression profiles in pancreatic ductal adenocarcinoma. Clinical implications

Age-related dermal collagen changes during development, maturation and ageing - a morphometric and comparative study.

The tissue organisation of dermal collagen is gaining importance as a contributing factor both in development and ageing, as well as in skin maturation processes. In this work we aim to study different representative parameters of this structural organisation in 45 human skin samples of assorted ages, by means of image analysis. The variation of these parameters on the basis of age was assessed using several regression models (linear, quadratic and cubic). The area occupied by collagen was significantly reduced as a function of age in the papillary dermis (R(2) = 0.437, P < 0.0001), as well as the thickness of the collagen bundles (R(2) = 0.461, P < 0.0001), following statistical models of …

Comparative measurement of collagen bundle orientation by Fourier analysis and semiquantitative evaluation: reliability and agreement in Masson's trichrome, Picrosirius red and confocal microscopy techniques

Summary Measurement of collagen bundle orientation in histopathological samples is a widely used and useful technique in many research and clinical scenarios. Fourier analysis is the preferred method for performing this measurement, but the most appropriate staining and microscopy technique remains unclear. Some authors advocate the use of Haematoxylin-Eosin (H&E) and confocal microscopy, but there are no studies comparing this technique with other classical collagen stainings. In our study, 46 human skin samples were collected, processed for histological analysis and stained with Masson's trichrome, Picrosirius red and H&E. Five microphotographs of the reticular dermis were taken with a 20…

Paraganglioma carotídeo maligno. A propósito de un caso

Resumen Los tumores del cuerpo carotideo son neoplasias poco frecuentes, de lento crecimiento, muy destructivos y vascularizados, que tienen un origen neuroectodermico. Los tumores malignos representan el 5-13% de los casos y presentan un comportamiento agresivo local con infiltracion de los ganglios linfaticos adyacentes o metastasis a distancia. Presentamos el caso de un varon de 60 anos al que en el estudio por dolor y parestesias en miembro superior se le realiza una resonancia magnetica donde se visualiza una gran tumoracion en el espacio carotideo izquierdo, la cual se biopsio obteniendo el diagnostico histologico de paraganglioma. Al realizar el estudio de extension se identifico la …

Malignant carotid paraganglioma: A case report

Tumors of the carotid body are uncommon neoplasms that originate in the neuroectoderm. These tumors are slow growing but well vascularized and very destructive. Between 5% and 13% of carotid body tumors are malignant, behaving aggressively at the local level, invading the adjacent lymph nodes, and resulting in remote metastases. We present the case of a 60-year-old man who was examined for pain and paresthesias in his arm. Magnetic resonance imaging showed a large tumor in the left carotid space. Histologic study of a biopsy specimen from the tumor revealed that it was a paraganglioma. Further studies to determine the extent of disease detected metastases in bone and lung, confirming the tu…