0000000000465494

AUTHOR

Klaus Eisele

showing 2 related works from this author

Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147.

2010

We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability.

MaleGreen Fluorescent ProteinsStatic ElectricitySus scrofaBiophysicsSerum albuminBioengineeringEndosomesBiologyTransfectionBiomaterialschemistry.chemical_compoundElectrolytesPlasmidEthidiumStatic electricityAnimalsHumansParticle SizeCell DeathAlbuminIsothermal titration calorimetrySerum Albumin BovineTransfectionDNAMiddle AgedPolyelectrolyteClathrinMolecular WeightchemistryBiochemistryMechanics of MaterialsSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationCeramics and CompositesBiophysicsbiology.proteinThermodynamicsDNAPlasmidsBiomaterials
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Cationized albumin-biocoatings for the immobilization of lipid vesicles

2010

Tethered lipid membranes or immobilized lipid vesicles are frequently used as biomimetic systems. In this article, the authors presented a suitable method for efficient immobilization of lipid vesicles onto a broad range of surfaces, enabling analysis by quantitative methods even under rigid, mechanical conditions-bare surfaces such as hydrophilic glass surfaces as well as hydrophobic polymer slides or metal surfaces such as gold. The immobilization of vesicles was based on the electrostatic interaction of zwitterionic or negatively charged lipid vesicles with two types of cationic chemically modified bovine serum albumin (cBSA) blood plasma proteins (cBSA-113 and cBSA-147). Quantitative an…

Chemistry(all)Surface PropertiesAnalytical chemistryGeneral Physics and AstronomyPhysics and Astronomy(all)Microscopy Atomic ForceGeneral Biochemistry Genetics and Molecular BiologyBiomaterialsCoated Materials BiocompatibleMaterials Science(all)CationsZeta potentialGeneral Materials ScienceBovine serum albuminSurface plasmon resonanceLiposomebiologyChemistryBiochemistry Genetics and Molecular Biology(all)VesicleSerum Albumin BovineGeneral ChemistryAdhesionMembraneLiposomesBiophysicsbiology.proteinAdsorptionProtein adsorptionProtein BindingBiointerphases
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