6533b820fe1ef96bd1279a18

RESEARCH PRODUCT

Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147.

Radu A. GropeanuKlaus EiseleHeiko J. LuhmannChristoph M. ZehendnerSubhash G. VasudevanGeorge MihovTanja WeilKaloian KoynovAli RouhanipourChristoph R.w. KuhlmannArvind Ramanathan

subject

MaleGreen Fluorescent ProteinsStatic ElectricitySus scrofaBiophysicsSerum albuminBioengineeringEndosomesBiologyTransfectionBiomaterialschemistry.chemical_compoundElectrolytesPlasmidEthidiumStatic electricityAnimalsHumansParticle SizeCell DeathAlbuminIsothermal titration calorimetrySerum Albumin BovineTransfectionDNAMiddle AgedPolyelectrolyteClathrinMolecular WeightchemistryBiochemistryMechanics of MaterialsSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationCeramics and CompositesBiophysicsbiology.proteinThermodynamicsDNAPlasmids

description

We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability.

yearjournalcountryeditionlanguage
2010-06-24Biomaterials
10.1016/j.biomaterials.2010.07.088https://pubmed.ncbi.nlm.nih.gov/20817248