0000000000465756
AUTHOR
Giuseppe Claudio Guarnera
Ad-Hoc Segmentation Pipeline for Microarray Image Analysis
Microarray is a new class of biotechnologies able to help biologist researches to extrapolate new knowledge from biological experiments. Image Analysis is devoted to extrapolate, process and visualize image information. For this reason it has found application also in Microarray, where it is a crucial step of this technology (e.g. segmentation). In this paper we describe MISP (Microarray Image Segmentation Pipeline), a new segmentation pipeline for Microarray Image Analysis. The pipeline uses a recent segmentation algorithm based on statistical analysis coupled with K-Means algorithm. The Spot masks produced by MISP are used to determinate spots information and quality measures. A software …
Adaptive Techniques for Microarray Image Analysis with Related Quality Assessment
We propose novel techniques for microarray image analysis. In particular, we describe an overall pipeline able to solve the most common problems of microarray image analysis. We pro- pose the microarray image rotation algorithm (MIRA) and the statis- tical gridding pipeline (SGRIP) as two advanced modules devoted to restoring the original microarray grid orientation and to detecting, the correct geometrical information about each spot of input mi- croarray, respectively. Both solutions work by making use of statis- tical observations, obtaining adaptive and reliable information about each spot property. They improve the performance of the microarray image segmentation pipeline (MISP) we rec…
A Novel Artificial Mosaic Generation Technique Driven by Local Gradient Analysis
Art often provides valuable hints for technological innovations especially in the field of Image Processing and Computer Graphics. In this paper we present a novel method to generate an artificial mosaic starting from a raster input image. This approach, based on Gradient Vector Flow computation and some smart heuristics, permit us to follow the most important edges maintaining at the same time high frequency details. Several examples and comparisons with other recent mosaic generation approaches show the effectiveness of our technique.