0000000000468543

AUTHOR

Mauro ŠTifanić

0000-0002-5412-0644

showing 2 related works from this author

Bio-vaterite formation by glycoproteins from freshwater pearls

2010

Abstract A 48 kDa acidic and putative calcium-binding glycoprotein was isolated from pearls of the freshwater mussel Hyriopsis cumingii . This protein was compared with a related 46 kDa polypeptide, obtained from the nacreous shell of the same species. Separation by two-dimensional gel electrophoresis revealed that the difference in molecular weight is due to the higher extent of glycosylation of the 48 kDa protein existing in pearls. Evidence is presented that the sugar moieties of the protein contribute to crystal growth, starting with the nucleation step. In in vitro precipitation experiments, the 48 kDa glycoprotein of pearls directed the formation of round-shaped vaterite crystals whil…

UnionidaeGlycosylationGlycosylationGeneral Physics and AstronomyFresh WaterBiologyCalcium Carbonatechemistry.chemical_compoundStructural BiologyVateriteAnimalsElectrophoresis Gel Two-DimensionalGeneral Materials ScienceCarbonic AnhydrasesGlycoproteinschemistry.chemical_classificationCalciteGel electrophoresisbiomineralization ; freshwater pearls ; calcium carbonate precipitation ; vaterite ; carbonic anhydraseCell BiologyMusselMolecular WeightCalcium carbonatechemistryBiochemistryGlycoproteinBiomineralization
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Expression of one sponge Iroquois homeobox gene in primmorphs from Suberites domuncula during canal formation

2003

SUMMARY Sponges (Porifera) represent the evolutionary oldest multicellular animals. They are provided with the basic molecules involved in cell–cell and cell–matrix interactions. We report here the isolation and characterization of a complementary DNA from the sponge Suberites domuncula coding for the sponge homeobox gene, SUBDOIRX-a. The deduced polypeptide with a predicted Mr of 44,375 possesses the highly conserved Iroquois-homeodomain. We applied in situ hybridization to localize Iroquois in the sponge. The expression of this gene is highest in cells adjacent to the canals of the sponge in the medulla region. To study the expression of Iroquois during development, the in vitro primmorph…

Molecular Sequence DataIn situ hybridizationFerric CompoundsComplementary DNAAnimalsCluster AnalysisGeneIn Situ HybridizationPhylogenyEcology Evolution Behavior and SystematicsDNA PrimersBase SequencebiologyMulticellular animalsGene Expression ProfilingGenes HomeoboxFresh-water sponge. Geodia-cydonium. Marine sponges. Ephydatia-muelleri. Adhesion receptors. Family. Origin. Cells. Identification. Evolution.Sequence Analysis DNAAnatomyBlotting Northernbiology.organism_classificationIn vitroPoriferaCell biologySuberites domunculaSpongeHomeoboxDevelopmental BiologyEvolution and Development
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