caracterization of a 4.6 kb upstream region necessary for the specific alfa2 neural tubulin gene expression in p. lividus embryos

A negative cis-regulatory module restrict Talfa2 tubulin gene expression in the neural territory of the sea urchin embryo.

A negative cis-regulatory module restrict Talfa2 tubulin gene expression in the neural territory of the sea urchin embryos

Cis regulation analysis of the sea urchin neural tubulin gene PlT¬alpha2

A Negative Cis-Regulatory Module Induces the Paracentrotus Lividus T?2 Gene Expression in Neurogenic Territory of the Embryo

identificazione del modulo di regolazione spaziale del gene talfa2 in paracentrotus lividus

Identificazione del modulo di regolazione spaziale del gene Tα2 in Paracentrotus lividus

A negative cis-regulatory module restrict Talpha2 tubulin gene expression in the neural territory of the sea urchin embryo.

Chromatin dynamics of the developmentally regulated P. lividus neural alpha tubulin gene

Over 40 years ago, Allfrey and colleagues (1964) suggested that two histone modifications, namely acetylation and methylation, might regulate RNA synthesis. Nowadays it is universally accepted that activation of gene expression strictly depends on enzymatic mechanisms able to dynamically modify chromatin structure. Here, using techniques including DNaseI hypersensitive site analysis, chomatin immunoprecipitation and quantitative PCR analysis, we have analyzed the dynamics of histone post-translation modifications involved in developmentally/spatially controlled activation of the sea urchin PlTalpha2 tubulin gene. We have demonstrated that only when the PlTalpha2 core promoter chromatin is a…

Analisi dell'espressione genica e dinamica della cromatinadel gene PITa2 in Paracentrotus Lividus.

Caracterization of a 4.6 Kb upstream region necessary for the specific a2 neural tubulin gene expression in P. lividus embryos.

A NEGATIVE CIS-REGULATORY MODULE INDUCES THE PARACENTROTUS LIVIDUS Talfa2 GENE EXPRESSION IN NEUROGENIC TERRITORY OF THE EMBRYO

Neural gene transcriptional regulation in sea urchin

A NEGATIVE CIS-REGULATORY MODULE INDUCES THE PARACENTROTUS LIVIDUS Talpha2 GENE EXPRESSION IN NEUROGENIC TERRITORY OF THE EMBRYO

I geni neurali di tubulina nello sviluppo di P. lividus

Analisi funzionale del promotore del gene Talfa2 in Paracentrotus lividus.

In silico characterization of the neural alpha tubulin gene promoter of the sea urchin embryo Paracentrotus lividus by phylogenetic footprinting

During Paracentrotus lividus sea urchin embryo development one alpha and one beta tubulin genes are expressed specifically in the neural cells and they are early end output of the gene regulatory network that specifies the neural commitment. In this paper we have used a comparative genomics approach to identify con- served regulatory elements in the P. lividus neural alpha tubulin gene. To this purpose, we have first isolated a genomic clone containing the entire gene plus 4.5 Kb of 5 0 upstream sequences. Then, we have shown by gene transfer experiments that its non-coding region drives the spatio- temporal gene expression corresponding substantially to that of the endogenous gene. In addi…

Transcriptional regulation of Tubulin α2 gene in Paracentrotus lividus.

The PlTα2 is a sea urchin Paracentrotus lividus gene coding for a neural isotype of alfa tubulin. Expression of Tα2 gene is temporally activated from the blastula stage and spatially confined to the neural territory of the embryo. In order to investigate cis-regulatory elements that control this spatio-temporal pattern we performed functional experiment microinjecting several constructs containing different portion of Tα2 promoter. Moreover quantitative chromatin immune-precipitation experiments were performed to evaluate a possible involvement of chromatin modifications in regulation of Tα2 gene expression.

Chromatin dynamics during sea urchin embryogenesis: effects on the neural alpha tubulin PlTa2 gene expression

Expression of PlTa2 gene during sea urchin P. lividus development, is spatially confined to the neural territory and temporally activated from the blastula stage. To evaluate a possible involvement of chromatin modifications in regulation of PlTa2 gene expression we first searched for DNaseI hypersentive sites. We found four sites localized in the introns of the gene, when we used chromatin extracted from embryos at gastrula stage but not from morula stage. This result suggests a possible functional role of the introns in the activation of the expression of PlTa2 gene. Moreover, we used specific antibodies for RNA polymerase II and for different modified form of lysine 9, lysine 27 and lysi…

Expression analysis and chromatin dynamics of tubulin PlT-alpha2 gene in Paracentrotus lividus.